Polyethylene glycol-conjugated pharmaceutical proteins

被引：229

作者：

Bailon, P ^{[1
]}

Berthold, W ^{[1
]}

机构：

[1] Hoffmann La Roche Inc, Dept Biopharmaceut, Nutley, NJ 07110 USA

来源：

PHARMACEUTICAL SCIENCE & TECHNOLOGY TODAY | 1998年 / 1卷 / 08期

关键词：

D O I：

10.1016/S1461-5347(98)00086-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Polyethylene glycol (PEG)-conjugated proteins belong to a new class of biomolecules that are neither proteins nor polymers, but which are hybrids of the two. PEG conjugation of biopharmaceuticals is now common practice in efforts to achieve sustained clinical response. However, very little is known about the strategies and criteria used to produce a well-characterized pegylated biomolecule. In this review, the issues of pegylation reagent selection, reaction conditions, process considerations and purification will be addressed, as well as biochemical and biological characterizations. The isolation of positional isomers and the determination of pegylation sites will also be included.

引用

页码：352 / 356

页数：7

共 59 条

[11] PROPERTIES OF 2 URATE OXIDASES MODIFIED BY THE COVALENT ATTACHMENT OF POLY(ETHYLENE GLYCOL) [J].

CHEN, RHL ;

ABUCHOWSKI, A ;

VANES, T ;

PALCZUK, NC ;

DAVIS, FF .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 660 (02) :293-298

[12]

DAVIS FF, 1980, BIOMEDICAL POLYM POL, P441

[13]

DAVIS S, 1981, CLIN EXP IMMUNOL, V46, P649

[14]

DELGADO C, 1992, CRIT REV THER DRUG, V9, P249

[15]

DELGADO C, 1990, BIOTECHNOL APPL BIOC, V12, P119

[16]

ELLING L, 1991, BIOTECHNOL APPL BIOC, V13, P354

[17] THE CLINICAL EFFICACY OF POLY(ETHYLENE GLYCOL)-MODIFIED PROTEINS [J].

FUERTGES, F ;

ABUCHOWSKI, A .

JOURNAL OF CONTROLLED RELEASE, 1990, 11 (1-3) :139-148

[18]

Fung W.-J., 1997, POLYM PREPRINT, V38, P565

[19] SITE-DIRECTED PEGYLATION OF RECOMBINANT INTERLEUKIN-2 AT ITS GLYCOSYLATION SITE [J].

GOODSON, RJ ;

KATRE, NV .

BIO-TECHNOLOGY, 1990, 8 (04) :343-346

[20] HIGHLY WATER-SOLUBLE TAXOL DERIVATIVES - 7-POLYETHYLENE GLYCOL CARBAMATES AND CARBONATES [J].

GREENWALD, RB ;

PENDRI, A ;

BOLIKAL, D .

JOURNAL OF ORGANIC CHEMISTRY, 1995, 60 (02) :331-336

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