A Flexible Approach to Studying Post-Transcriptional Gene Regulation in Stably Transfected Mammalian Cells

被引：5
作者：
Nichols, Ralph C. [1 ,2 ]
Botson, John [2 ]
Wang, Xiao Wei
Hamilton, B. JoNell
Collins, Jane E.
Uribe, Victoria
Brooks, Seth A. [1 ,2 ]
Zan, Moe [2 ]
Rigby, William F. C. [2 ]
机构：
[1] Vet Affairs Med Ctr, Vet Adm Res Serv, White River Jct, VT 05009 USA
[2] Dartmouth Med Sch, Dept Med, Lebanon, NH 03756 USA
来源：
MOLECULAR BIOTECHNOLOGY | 2011年 / 48卷 / 03期
基金：
美国国家卫生研究院;
关键词：
ARE; TNF; mRNA turnover; Translation; 3 '-UTR; ACTIVATED PROTEIN-KINASE; MESSENGER-RNA DEGRADATION; TNF-ALPHA; LIPOPOLYSACCHARIDE STIMULATION; TRISTETRAPROLIN TTP; TRANSCRIPTION; EXPRESSION; TRANSLATION; BIOSYNTHESIS; INDUCTION;
D O I：
10.1007/s12033-010-9360-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The study of post-transcriptional regulation is constrained by the technical limitations associated with both transient and stable transfection of chimeric reporter plasmids examining the activity of 3'-UTR cis-acting elements. We report the adaptation of a commercially available system that enables consistent stable integration of chimeric reporter cDNA into a single genomic site in which transcription is induced by tetracycline. Using this system, we demonstrate the tight control afforded by this system and its suitability in mapping the regulatory function of defined cis-acting elements in the human TNF 3'-UTR, as well as the distinct effects of serum starvation on transiently transfected and stably integrated chimeric reporter genes.
引用
收藏
页码：210 / 217
页数：8
相关论文
共 50 条
  • [31] Zinc triggers a complex transcriptional and post-transcriptional regulation of the metal homeostasis gene FRD3 in Arabidopsis relatives
    Charlier, Jean-Benoit
    Polese, Catherine
    Nouet, Cecile
    Carnol, Monique
    Bosman, Bernard
    Kraemer, Ute
    Motte, Patrick
    Hanikenne, Marc
    JOURNAL OF EXPERIMENTAL BOTANY, 2015, 66 (13) : 3865 - 3878
  • [32] Post-transcriptional regulation by cytosine-5 methylation of RNA
    Garcia-Vilchez, Raquel
    Sevilla, Ana
    Blanco, Sandra
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2019, 1862 (03): : 240 - 252
  • [33] Post-transcriptional gene regulation: From genome-wide studies to principles
    Halbeisen, R. E.
    Galgano, A.
    Scherrer, T.
    Gerber, A. P.
    CELLULAR AND MOLECULAR LIFE SCIENCES, 2008, 65 (05) : 798 - 813
  • [34] Post-transcriptional regulation of antiviral gene expression by N6-methyladenosine
    McFadden, Michael J.
    McIntyre, Alexa B. R.
    Mourelatos, Haralambos
    Abell, Nathan S.
    Gokhale, Nandan S.
    Ipas, Helene
    Xhemalce, Blerta
    Mason, Christopher E.
    Horner, Stacy M.
    CELL REPORTS, 2021, 34 (09):
  • [35] Post-transcriptional regulation of cytokine and growth factor signaling in cancer
    Louis, Irina Vlasova-St.
    Bohjanen, Paul R.
    CYTOKINE & GROWTH FACTOR REVIEWS, 2017, 33 : 83 - 93
  • [36] The role of CEMIP in cancers and its transcriptional and post-transcriptional regulation
    Guo, Song
    Guo, Yunfei
    Chen, Yuanyuan
    Cui, Shuaishuai
    Zhang, Chunmei
    Chen, Dahu
    PEERJ, 2024, 12
  • [37] Post-transcriptional gene regulation: From genome-wide studies to principles
    R. E. Halbeisen
    A. Galgano
    T. Scherrer
    A. P. Gerber
    Cellular and Molecular Life Sciences, 2008, 65
  • [38] Transcriptional and Post-transcriptional Gene Regulation by Long Non-coding RNA
    Dykes, Iain M.
    Emanueli, Costanza
    GENOMICS PROTEOMICS & BIOINFORMATICS, 2017, 15 (03) : 177 - 186
  • [39] RISC assembly and post-transcriptional gene regulation in Hepatocellular Carcinoma
    Santhekadur, Prasanna K.
    Kumar, Divya P.
    GENES & DISEASES, 2020, 7 (02) : 199 - 204
  • [40] Post-transcriptional gene regulation in the biology and virulence of Candida albicans
    Verma-Gaur, Jiyoti
    Traven, Ana
    CELLULAR MICROBIOLOGY, 2016, 18 (06) : 800 - 806
12345