RETRACTED: Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis (Retracted Article)

被引：21
作者：
Sun, Ting [1 ]
Liu, Zhexian [2 ]
Zhang, Rui [2 ]
Ma, Siping [2 ]
Lin, Tao [2 ]
Li, Yanxi [2 ]
Yang, Shihua [2 ]
Zhang, Wanchuan [2 ]
Wang, Yongpeng [2 ]
机构：
[1] China Med Univ, Liaoning Canc Hosp & Inst, Dept Blood Transfus, Canc Hosp, Shenyang 110042, Peoples R China
[2] China Med Univ, Liaoning Canc Hosp & Inst, Canc Hosp, Dept Colorectal Surg, 44 Xiaoheyan Rd, Shenyang 110042, Peoples R China
来源：
ONCOTARGETS AND THERAPY | 2020年 / 13卷
关键词：
LEF1-AS1; colon cancer; miR-30-5p; SOX9; COLORECTAL-CANCER; TUMOR-SUPPRESSOR; SOX9; EXPRESSION; GENE; PROLIFERATION; GENOMICS;
D O I：
10.2147/OTT.S232839
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Introduction: Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in the tumorigenesis and progression of colon cancer. Lymphoid enhancer-binding factor 1 antisense RNA 1 (LEF1-AS1), a highly conserved and newly discovered long noncoding RNA, has been reported to be upregulated and correlated with poor prognosis in colon cancer, but the exact role of it remains uncertain. Materials and Methods: In our study, the biological functions of LEF1-AS1 in colon cancer were analyzed by cell viability assay, colony formation assay, scratch wound healing assay, transwell cell invasion assay, soft agar assay, luciferase reporter assay, pull down assay, tumor xenograft model and Western blot. Results: We found that LEF1-AS1 was upregulated in colon cancer patients and correlated with poor overall survival and recurrent-free survival. Besides, enforced expression of LEF1-AS1 in HT29 and T84 cells promoted migration, invasion, anchorage-independent growth, tumor xenograft formation and lung metastasis, while knockdown of LEF1-AS1 in COLO320 cells suppressed cell migration, invasion, anchorage-independent growth and tumor xenograft formation. In addition, LEF1-AS1 was directly interacted and inversely correlated with miR-30-5p in colon cancer, and SOX9 was a downstream target for miR-30-5p. LEF1-AS1 overexpression increased the expression level of SOX9, and restoration of SOX9 attenuated the effects caused by LEF1-AS1 knockdown in cell migration, invasion, anchorage-independent growth and tumor xenograft formation. Conclusion: Our results indicated that LEF1-AS1 promotedmigration, invasion and metastasis of colon cancer cells partially through miR-30-5p/SOX9 axis. The oncogenic LEF1-AS1 could be a potential prognostic biomarker for colon cancer.
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收藏
页码：2957 / 2972
页数：16
相关论文
共 45 条
[1]   MiniSOX9, a dominant-negative variant in colon cancer cells [J].
Abdel-Samad, R. ;
Zalzali, H. ;
Rammah, C. ;
Giraud, J. ;
Naudin, C. ;
Dupasquier, S. ;
Poulat, F. ;
Boizet-Bonhoure, B. ;
Lumbroso, S. ;
Mouzat, K. ;
Bonnans, C. ;
Pignodel, C. ;
Raynaud, P. ;
Fort, P. ;
Quittau-Prevostel, C. ;
Blache, P. .
ONCOGENE, 2011, 30 (22) :2493-2503
[2]   Outcome prediction and the future of the TNM staging system [J].
Burke, HB .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2004, 96 (19) :1408-1409
[3]   SOX9-regulated cell plasticity in colorectal metastasis is attenuated by rapamycin [J].
Carrasco-Garcia, Estefania ;
Lopez, Lidia ;
Aldaz, Paula ;
Arevalo, Sara ;
Aldaregia, Juncal ;
Egana, Larraitz ;
Bujanda, Luis ;
Cheung, Martin ;
Sampron, Nicolas ;
Garcia, Idoia ;
Matheu, Ander .
SCIENTIFIC REPORTS, 2016, 6
[4]   Worldwide Variations in Colorectal Cancer [J].
Center, Melissa M. ;
Jemal, Ahmedin ;
Smith, Robert A. ;
Ward, Elizabeth .
CA-A CANCER JOURNAL FOR CLINICIANS, 2009, 59 (06) :366-378
[5]   The cBio Cancer Genomics Portal: An Open Platform for Exploring Multidimensional Cancer Genomics Data [J].
Cerami, Ethan ;
Gao, Jianjiong ;
Dogrusoz, Ugur ;
Gross, Benjamin E. ;
Sumer, Selcuk Onur ;
Aksoy, Buelent Arman ;
Jacobsen, Anders ;
Byrne, Caitlin J. ;
Heuer, Michael L. ;
Larsson, Erik ;
Antipin, Yevgeniy ;
Reva, Boris ;
Goldberg, Arthur P. ;
Sander, Chris ;
Schultz, Nikolaus .
CANCER DISCOVERY, 2012, 2 (05) :401-404
[6]   A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNA [J].
Cesana, Marcella ;
Cacchiarelli, Davide ;
Legnini, Ivano ;
Santini, Tiziana ;
Sthandier, Olga ;
Chinappi, Mauro ;
Tramontano, Anna ;
Bozzoni, Irene .
CELL, 2011, 147 (02) :358-369
[7]   Identification of dysregulated lncRNAs profiling and metastasis-associated lncRNAs in colorectal cancer by genome-wide analysis [J].
Chen, Yan ;
Yu, Xiang ;
Xu, Yongcan ;
Shen, Hua .
CANCER MEDICINE, 2017, 6 (10) :2321-2330
[8]   Long non-coding RNAs: New biomarkers for prognosis and diagnosis of colon cancer [J].
Deng, Heng ;
Wang, Jian Min ;
Li, Ming ;
Tang, Ran ;
Tang, Kun ;
Su, Yingzi ;
Hou, Yong ;
Zhang, Jun .
TUMOR BIOLOGY, 2017, 39 (06)
[9]   Distinct SOX9 levels differentially mark stem/progenitor populations and enteroendocrine cells of the small intestine epithelium [J].
Formeister, Eric J. ;
Sionas, Ayn L. ;
Lorance, David K. ;
Barkley, Carey L. ;
Lee, Ginny H. ;
Magness, Scott T. .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2009, 296 (05) :G1108-G1118
[10]   Long-range regulation at the SOX9 locus in development and disease [J].
Gordon, C. T. ;
Tan, T. Y. ;
Benko, S. ;
FitzPatrick, D. ;
Lyonnet, S. ;
Farlie, P. G. .
JOURNAL OF MEDICAL GENETICS, 2009, 46 (10) :649-656
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