A Polymer-based Monolithic Capillary Column with Polymyxin-B Chiral Selector for the Enantioselective Nano-High Performance Liquid Chromatographic Pharmaceutical Analysis * , **

被引：13

作者：

Ghanem, Ashraf ^{[1
]}

Marzouk, Adel A. ^{[2
]}

El-Adl, Sobhy M. ^{[3
]}

Fouad, Ali ^{[1
,2
]}

机构：

[1] Univ Canberra, Fac Sci & Technol, Chiral Program, Canberra, ACT 2601, Australia

[2] Al Azhar Univ, Fac Pharm, Pharmaceut Chem Dept, Assiut 71524, Egypt

[3] Zagazig Univ, Fac Pharm, Pharmaceut Chem Dept, Zagazig 44519, Egypt

来源：

JOURNAL OF CHROMATOGRAPHY A | 2022年 / 1662卷

关键词：

Polymyxin-B; Monolith; Organic polymer; Enantio-separation; Encapsulation; Capillary; SILICA STATIONARY-PHASE; REVERSED-PHASE; MACROCYCLIC ANTIBIOTICS; SEPARATION; ELECTROCHROMATOGRAPHY; ENANTIOSEPARATION; HPLC; ENANTIOMERS; EREMOMYCIN; STABILITY;

D O I：

10.1016/j.chroma.2021.462714

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein, we report the first use of Polymyxin-B antibiotic as a enantio-selector in polymer monolithic capillary. The capillaries were functionalised, characterized and tested for the enantioselective nanoHPLC separation of 50 racemic pharmaceutical drugs. They have been easily prepared by immobilizing Polymyxin-B over the organic polymer for 48 h (P1) or encapsulating Polymyxin-B within the organic polymer (P2) and tested for the enantioselective resolution of racemic drugs. Acceptable resolution was achieved for 21 drugs using RP-HPLC conditions on both (P1) and (P2) capillary columns, while no separation was observed under NP-HPLC conditions. Polymyxin-B is commercially available, easily solubilized and stable in both acidic and neutral media. The developed Polymyxin-B-based polymer monolithic capillaries provide a promising expansion of platform in enantioselective HPLC separations. (c) 2021 Elsevier B.V. All rights reserved.

引用

页数：10

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