Toward characterizing extracellular vesicles at a single-particle level

被引：120

作者：

Chiang, Chun-yi ^{[1
]}

Chen, Chihchen ^{[1
,2
]}

机构：

[1] Natl Tsing Hua Univ, Inst Nanoengn & Microsyst, Hsinchu 30013, Taiwan

[2] Natl Tsing Hua Univ, Dept Power Mech Engn, Hsinchu, Taiwan

来源：

JOURNAL OF BIOMEDICAL SCIENCE | 2019年 / 26卷 / 1期

关键词：

Extracellular vesicle; Single particle; Exosome; Microvesicle; Electrophoresis; Raman spectroscopy; Atomic force microscopy; Digital PCR; RAMAN-SPECTROSCOPY; ELECTRON-MICROSCOPY; EXOSOME ISOLATION; CANCER; CELLS; MICROPARTICLES; MICRORNAS; PROTEIN; PLASMA; IDENTIFICATION;

D O I：

10.1186/s12929-019-0502-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles that serve a means of cell-cell communication. Studying EVs at a single-particle level is important because EVs are inherently heterogeneous. Novel micro- and nanotechnological tools have open opportunities for realizing single-EV measurements exploiting their biochemical, electrical, mechanical, and/or optical properties. This review summarizes the recent development of technologies toward sorting and analyzing single EVs. Sorting EVs into a more homogeneous subset relaxes the sensitivity and throughput required on the EV detection, and hence related techniques are also included in this review. These exciting technologies are on the rise and will expand our understanding of EVs and their applications in the near future.

引用

页数：10

共 95 条

[81] Optical and non-optical methods for detection and characterization of microparticles and exosomes [J].

van der Pol, E. ;

Hoekstra, A. G. ;

Sturk, A. ;

Otto, C. ;

van Leeuwen, T. G. ;

Nieuwland, R. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2010, 8 (12) :2596-2607

[82] Classification, Functions, and Clinical Relevance of Extracellular Vesicles [J].

van der Pol, Edwin ;

Boing, Anita N. ;

Harrison, Paul ;

Sturk, Augueste ;

Nieuwland, Rienk .

PHARMACOLOGICAL REVIEWS, 2012, 64 (03) :676-705

[83] Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry [J].

van der Vlist, Els J. ;

Nolte-'t Hoen, Esther N. M. ;

Stoorvogel, Willem ;

Arkesteijn, Ger J. A. ;

Wauben, Marca H. M. .

NATURE PROTOCOLS, 2012, 7 (07) :1311-1326

[84] MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins [J].

Vickers, Kasey C. ;

Palmisano, Brian T. ;

Shoucri, Bassem M. ;

Shamburek, Robert D. ;

Remaley, Alan T. .

NATURE CELL BIOLOGY, 2011, 13 (04) :423-U182

[85] Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles [J].

Wang, Zongxing ;

Wu, Hung-jen ;

Fine, Daniel ;

Schmulen, Jeffrey ;

Hu, Ye ;

Godin, Biana ;

Zhang, John X. J. ;

Liu, Xuewu .

LAB ON A CHIP, 2013, 13 (15) :2879-2882

[86] Digital microfluidics-enabled single-molecule detection by printing and sealing single magnetic beads in femtoliter droplets [J].

Witters, Daan ;

Knez, Karel ;

Ceyssens, Frederik ;

Puers, Robert ;

Lammertyn, Jeroen .

LAB ON A CHIP, 2013, 13 (11) :2047-2054

[87] Standardization of sample collection, isolation and analysis methods in extracellular vesicle research [J].

Witwer, Kenneth W. ;

Buzas, Edit I. ;

Bemis, Lynne T. ;

Bora, Adriana ;

Lasser, Cecilia ;

Lotvall, Jan ;

Hoen, Esther N. Nolte-'t ;

Piper, Melissa G. ;

Sivaraman, Sarada ;

Skog, Johan ;

Thery, Clotilde ;

Wauben, Marca H. ;

Hochberg, Fred .

JOURNAL OF EXTRACELLULAR VESICLES, 2013, 2 (01)

[88] NATURE AND SIGNIFICANCE OF PLATELET PRODUCTS IN HUMAN PLASMA [J].

WOLF, P .

BRITISH JOURNAL OF HAEMATOLOGY, 1967, 13 (03) :269-+

[89] Effect of exosome isolation methods on physicochemical properties of exosomes and clearance of exosomes from the blood circulation [J].

Yamashita, Takuma ;

Takahashi, Yuki ;

Nishikawa, Makiya ;

Takakura, Yoshinobu .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2016, 98 :1-8

[90] Biological properties of extracellular vesicles and their physiological functions [J].

Yanez-Mo, Maria ;

Siljander, Pia R. -M. ;

Andreu, Zoraida ;

Zavec, Apolonija Bedina ;

Borras, Francesc E. ;

Buzas, Edit I. ;

Buzas, Krisztina ;

Casal, Enriqueta ;

Cappello, Francesco ;

Carvalho, Joana ;

Colas, Eva ;

Cordeiro-da Silva, Anabela ;

Fais, Stefano ;

Falcon-Perez, Juan M. ;

Ghobrial, Irene M. ;

Giebel, Bernd ;

Gimona, Mario ;

Graner, Michael ;

Gursel, Ihsan ;

Gursel, Mayda ;

Heegaard, Niels H. H. ;

Hendrix, An ;

Kierulf, Peter ;

Kokubun, Katsutoshi ;

Kosanovic, Maja ;

Kralj-Iglic, Veronika ;

Kraemer-Albers, Eva-Maria ;

Laitinen, Saara ;

Lasser, Cecilia ;

Lener, Thomas ;

Ligeti, Erzsebet ;

Line, Aija ;

Lipps, Georg ;

Llorente, Alicia ;

Lotvall, Jan ;

Mancek-Keber, Mateja ;

Marcilla, Antonio ;

Mittelbrunn, Maria ;

Nazarenko, Irina ;

Nolte-t' Hoen, Esther N. M. ;

Nyman, Tuula A. ;

O'Driscoll, Lorraine ;

Olivan, Mireia ;

Oliveira, Carla ;

Pallinger, Eva ;

del Portillo, Hernando A. ;

Reventos, Jaume ;

Rigau, Marina ;

Rohde, Eva ;

Sammar, Marei .

JOURNAL OF EXTRACELLULAR VESICLES, 2015, 4 :1-60

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