A Modified Ink Staining Procedure for Cryptococcus Detection of the Cerebrospinal Fluid by Microscope

被引：4

作者：

Wang, Hua-Cheng ^{[1
]}

Ouyang, Ya-Fang ^{[1
]}

Wang, Li-Na ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 7, Clin Neurosci Ctr, 628 Zhenyuan Rd, Shenzhen 518107, Guangdong, Peoples R China

[2] Guangdong Prov Hosp TDM, Dept Clin Lab, Guangzhou, Peoples R China

来源：

CLINICAL LABORATORY | 2021年 / 67卷 / 12期

关键词：

meningoencephalitis; cryptococcus; cell slide centrifuge; cerebrospinal fluid;

D O I：

10.7754/Clin.Lab.2021.210732

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The pathogen detection rate of traditional ink staining procedure (TISP) is low. Methods: A modified ink staining procedure (MISP) was created to accumulate pathogen by using cell slide centrifuge. Items of RBC, WBC, and cryptococcus were observed. Results: There were 79.98 +/- 54.94 RBC and 126.98 +/- 36.39 WBC in one MISP microscopic field whereas there were only 3.35 +/- 2.41 RBC and 6.15 +/- 1.85 WBC in one TISP microscopic field in the same sample (*200). There was statistical difference between those two methods (p = 0.000). There were 40.78 +/- 13.23 mL cryptococcus in CSF processed by MISP whereas there were only 2.10 +/- 1.10 mL cryptococcus in the same CSF processed by TISP. There was statistical difference between those two methods. Conclusions: The modified ink staining procedure contributes to the separation of cells and pathogens (such as cryptococcus).

引用

页码：2721 / 2725

页数：5

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