Photoactive RuII-Polypyridyl Complexes that Display Sequence Selectivity and High-Affinity Binding to Duplex DNA through Groove Binding

被引：58

作者：

Ghosh, Amrita ^{[2
]}

Das, Priyadip ^{[2
]}

Gill, Martin R. ^{[1
]}

Kar, Prasenjit ^{[2
]}

Walker, Michaell G. ^{[1
]}

Thomas, Jim A. ^{[1
]}

Das, Amitava ^{[2
]}

机构：

[1] Univ Sheffield, Dept Chem, Sheffield S3 7HF, S Yorkshire, England

[2] Cent Salt & Marine Chem Res Inst, CSIR, Bhavnagar 364002, Gujarat, India

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 07期

基金：

英国工程与自然科学研究理事会;

关键词：

DNA; groove binding; luminescence; molecular recognition; ruthenium; DINUCLEAR RUTHENIUM(II) COMPLEX; MIXED-LIGAND COMPLEXES; ELECTRON-TRANSFER; LIGHT-SWITCH; MINOR-GROOVE; CRYSTAL-STRUCTURE; NUCLEIC-ACIDS; METAL-COMPLEXES; EXCITED-STATE; X-RAY;

D O I：

10.1002/chem.201002149

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The duplex-DNA binding properties of a nonintercalating polypyridyl ruthenium(II) complex that incorporates a linear extended ligand with a catechol moiety has been probed with a variety of photo- and biophysical techniques. These studies reveal that the complex groove binds to DNA sequences biphasically, and displays binding constants equivalent to those of high-affinity metallointercalators. The complex also displays preferential binding to AT-rich sequences. Changes in the structure of the coordinated catechol ligand and the incorporation of intercalating ancillary ligands into the complex were found to modulate both the optical-binding response and binding parameters of the system, which indicates that the catechol moiety plays a crucial role in the observed enhancement to binding affinities.

引用

页码：2089 / 2098

页数：10

共 28 条

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