ETS1 Ameliorates Hyperoxia-Induced Alveolar Epithelial Cell Injury by Regulating the TGM2-Mediated Wnt/β-Catenin Pathway

被引:10
|
作者
Yang, Min [1 ]
Gao, Xi-Rong [2 ]
Meng, Yan-Ni [1 ]
Shen, Fang [3 ]
Chen, Yan-Ping [1 ]
机构
[1] Hunan Childrens Hosp, Dept Resp, 86 Ziyuan Rd, Changsha 410007, Hunan, Peoples R China
[2] Hunan Childrens Hosp, Neonate Dept, Changsha 410007, Hunan, Peoples R China
[3] Hunan Childrens Hosp, Res Inst Children, Changsha 410007, Hunan, Peoples R China
基金
湖南省自然科学基金;
关键词
Bronchopulmonary dysplasia; ETS1; TGM2; TRANSGLUTAMINASE; 2; BRONCHOPULMONARY DYSPLASIA; EXPRESSION; CANCER; ANGIOGENESIS;
D O I
10.1007/s00408-021-00489-9
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Purpose Bronchopulmonary dysplasia (BPD) is a chronic lung disease that affects newborns who need oxygen therapy, and high-concentration oxygen therapy may cause neonatal morbidity and mortality in newborns. E26 oncogene homologue 1 (ETS1) and transglutaminase 2 (TGM2) have been reported to be associated with lung cell injury. However, the mechanism of ETS1 in regulating BPD is still unclear. Methods Hyperoxia-induced A549 cells to simulate hyperoxia-induced alveolar epithelial cell injury. MTT assays and colony formation assays were performed to investigate the proliferation of A549 cells. Flow cytometry was carried out to quantify the apoptosis of A549 cells. The expression levels of ETS1 and TGM2 were quantified by qRT-PCR. The protein expression levels of ETS1, TGM2, beta-catenin, c-Jun and MET were measured by western blot. Overexpression of ETS1, overexpression of TGM2, overexpression of ETS1 with downregulation of TGM2 and overexpression of TGM2 with inhibition of Wnt/beta-catenin pathway were performed to investigate the role of ETS1, TGM2 and Wnt/beta-catenin pathways in hyperoxia-induced alveolar epithelial cell injury. Results Hyperoxia decreased the proliferation and promoted the apoptosis of cells in a time-dependent manner. Moreover, overexpression of ETS1 rescued the effect of hyperoxia on proliferation and apoptosis. In addition, overexpression of TGM2 participated in the regulation of hyperoxia-induced proliferation and apoptosis. ETS1 regulated hyperoxia-induced alveolar epithelial cell injury through the Wnt/beta-catenin pathway via TGM2. Conclusion ETS1 ameliorates hyperoxia-induced alveolar epithelial cell injury through the TGM2-mediated Wnt/beta-catenin pathway.
引用
收藏
页码:681 / 690
页数:10
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