Goat's Milk as a Potential Anti-proliferative against Colon Cancer Cell Lines

被引:1
|
作者
Dahlan, Nurhashimah [1 ]
Baharum, Jamila Khayrin [1 ]
Ridzwan, Norhaslinda [1 ]
Jumli, Mimie Noratiqah [1 ]
Abd Hadi, Norhayati [1 ]
Arshad, Roslan [2 ]
Baig, Atif Amin [3 ]
Rohin, Mohd Adzim Khalili [1 ]
Abu Bakar, Che Abdullah [2 ]
Latif, Ahmad Zubaidi A. [3 ]
机构
[1] Univ Sultan Zainal Abidin, Fac Hlth Sci, Sch Nutr & Dietet, Gong Badak Campus, Kuala Nerus 21300, Terengganu Daru, Malaysia
[2] Univ Sultan Zainal Abidin, Fac Bioresources & Food Ind, Besut Campus, Besut 22200, Terengganu Daru, Malaysia
[3] Univ Sultan Zainal Abidin, Fac Med, Med Campus,Jalan Sultan Mahmud, Kuala Terengganu 20400, Terengganu Daru, Malaysia
关键词
Anti-proliferative; goat milk; colon cancer; MTT assay; FATTY-ACIDS; APOPTOSIS;
D O I
10.9734/JPRI/2021/v33i44A32642
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Aim: To investigate anti-proliferative effect of fresh and pasteurized goat milk against colon cancer cell lines (HT-29, HCT-116, CT-26). Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between January 2020 and April 2020. Methodology: Samples comprised of two types goat milk, which were fresh and pasteurized in powder form. The samples were analysed for the anti-proliferative effect by MTT assay, and IC50 value was determined. Then, cell apoptotic changes were observed by light inverted microscope by 24, 48 and 72 hours. Results: Experimental data showed that the fresh sample produce the highest yield (9.40%) than the pasteurized sample (7.17%). The fresh sample yielded the most potent cytotoxic value (0.28 +/- 0.03), followed by pasteurized sample with value IC50 0.32 +/- 0.02 against HCT-116 cells. Then, the anti-proliferative effect was observed on cell apoptotic changes by reduction of cell volume, cell densed, and presence of fragmentation and apoptotic bodies at 24, 48 and 72 hours treatment. Conclusion: In conclusion, the fresh sample of goat milk yielded the potent anti-proliferative effect than pasteurized sample.
引用
收藏
页码:493 / 501
页数:9
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