Goat's Milk as a Potential Anti-proliferative against Colon Cancer Cell Lines

被引：1

作者：

Dahlan, Nurhashimah ^{[1
]}

Baharum, Jamila Khayrin ^{[1
]}

Ridzwan, Norhaslinda ^{[1
]}

Jumli, Mimie Noratiqah ^{[1
]}

Abd Hadi, Norhayati ^{[1
]}

Arshad, Roslan ^{[2
]}

Baig, Atif Amin ^{[3
]}

Rohin, Mohd Adzim Khalili ^{[1
]}

Abu Bakar, Che Abdullah ^{[2
]}

Latif, Ahmad Zubaidi A. ^{[3
]}

机构：

[1] Univ Sultan Zainal Abidin, Fac Hlth Sci, Sch Nutr & Dietet, Gong Badak Campus, Kuala Nerus 21300, Terengganu Daru, Malaysia

[2] Univ Sultan Zainal Abidin, Fac Bioresources & Food Ind, Besut Campus, Besut 22200, Terengganu Daru, Malaysia

[3] Univ Sultan Zainal Abidin, Fac Med, Med Campus,Jalan Sultan Mahmud, Kuala Terengganu 20400, Terengganu Daru, Malaysia

来源：

JOURNAL OF PHARMACEUTICAL RESEARCH INTERNATIONAL | 2021年 / 33卷 / 44A期

关键词：

Anti-proliferative; goat milk; colon cancer; MTT assay; FATTY-ACIDS; APOPTOSIS;

D O I：

10.9734/JPRI/2021/v33i44A32642

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Aim: To investigate anti-proliferative effect of fresh and pasteurized goat milk against colon cancer cell lines (HT-29, HCT-116, CT-26). Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between January 2020 and April 2020. Methodology: Samples comprised of two types goat milk, which were fresh and pasteurized in powder form. The samples were analysed for the anti-proliferative effect by MTT assay, and IC50 value was determined. Then, cell apoptotic changes were observed by light inverted microscope by 24, 48 and 72 hours. Results: Experimental data showed that the fresh sample produce the highest yield (9.40%) than the pasteurized sample (7.17%). The fresh sample yielded the most potent cytotoxic value (0.28 +/- 0.03), followed by pasteurized sample with value IC50 0.32 +/- 0.02 against HCT-116 cells. Then, the anti-proliferative effect was observed on cell apoptotic changes by reduction of cell volume, cell densed, and presence of fragmentation and apoptotic bodies at 24, 48 and 72 hours treatment. Conclusion: In conclusion, the fresh sample of goat milk yielded the potent anti-proliferative effect than pasteurized sample.

引用

页码：493 / 501

页数：9

共 35 条

[1] Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid inhibits G1-S progression in HT-29 human colon cancer cells [J].