Selection of reference gene from Gracilaria lemaneiformis under temperature stress

被引：24

作者：

Ding, Yan ^{[1
]}

Sun, Hengyi ^{[1
]}

Zhang, Ran ^{[1
]}

Yang, Qin ^{[1
]}

Liu, Yuantao ^{[1
]}

Zang, Xiaonan ^{[1
]}

Zhang, Xuecheng ^{[1
]}

机构：

[1] Ocean Univ China, Minist Educ, Key Lab Marine Genet & Breeding, Qingdao 266003, Shandong, Peoples R China

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2015年 / 27卷 / 03期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Reference gene; RT-qPCR; Gracilaria lemaneiformis; Temperature; TIME RT-PCR; ENDOGENOUS REFERENCE GENES; EXPRESSION; NORMALIZATION; GROWTH; MODEL; VALIDATION; BLADDER; CANCER;

D O I：

10.1007/s10811-014-0423-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Selection of a reliable reference gene for the normalization of RT-qPCR data is important to obtain accurate results in the study of gene expression. To elucidate the effects of temperature on the gene expression in Gracilaria lemaneiformis, this study aimed to find an appropriate reference gene from ten candidate genes, including actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 (EF1), eukaryotic translation initiation factor (eIF), histone (H2B), 18S ribosomal rRNA (18S rRNA), internal transcribed spacer 2 (ITS2), cytochrome c oxidase (COX), cytochrome b-5 reductase (CR) and phycoerythrin (PEB) of G. lemaneiformis under temperature stress. Programs geNorm and NormFinder were used to analyze the data of RT-qPCR. The results showed that the best reference gene combination was eIF, ACT for 32A degrees C different treatments, GAPDH, ITS2, CR, and 18SrRNA for 8 A degrees C different treatments, GAPDH, EF1 and ACT for different temperature treatments, and GAPDH and ACT for different life generations. This study provides the useful reference genes for future gene expression studies of G. lemaneiformis under temperature stress.

引用

页码：1365 / 1372

页数：8

共 26 条

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