Iwr1 Directs RNA Polymerase II Nuclear Import

被引：66
作者：
Czeko, Elmar [1 ,2 ]
Seizl, Martin [1 ,2 ]
Augsberger, Christian [1 ,2 ]
Mielke, Thorsten [3 ]
Cramer, Patrick [1 ,2 ]
机构：
[1] Univ Munich, Gene Ctr, D-81377 Munich, Germany
[2] Univ Munich, Ctr Integrated Prot Sci Munich CIPSM, Dept Biochem, D-81377 Munich, Germany
[3] Max Planck Inst Mol Genet, Ultrastrukturnetzwerk, D-14195 Berlin, Germany
来源：
MOLECULAR CELL | 2011年 / 42卷 / 02期
关键词：
SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; MEDIATOR SUBMODULE; PROTEIN COMPLEXES; STRUCTURAL BASIS; CARGO-BINDING; YEAST; TRANSCRIPTION; SUBUNITS; REVEALS;
D O I：
10.1016/j.molcel.2011.02.033
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
RNA polymerase (Pol) II transcribes protein-coding genes in the nucleus of eukaryotic cells and consists of 12 polypeptide subunits. It is unknown how Pol II is imported into the nucleus. Here we show that Pol II nuclear import requires the protein Iwr1 and provide evidence for cyclic Iwr1 function. Iwr1 binds Pol II in the active center cleft between the two largest subunits, maybe facilitating or sensing complete Pol II assembly in the cytoplasm. Iwr1 then uses an N-terminal bipartite nuclear localization signal that is recognized by karyopherin alpha to direct Pol II nuclear import. In the nucleus, Iwr1 is displaced from Pol II by transcription initiation factors and nucleic acids, enabling its export and recycling. Iwr1 function is Pol II specific, transcription independent, and apparently conserved from yeast to human.
引用
收藏
页码：261 / 266
页数：6
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