Organogallium(III) complexes as apoptosis promoting anticancer agents for head and neck squamous cell carcinoma (HNSCC) cell lines

被引：20

作者：

Kaluderovic, Milena R. ^{[2
]}

Kaluderovic, Goran N. ^{[1
,3
]}

Gomez-Ruiz, Santiago ^{[4
]}

Paschke, Reinhard ^{[1
]}

Hemprich, Alexander ^{[2
]}

Kuehling, Jan ^{[5
]}

Remmerbach, Torsten W. ^{[2
,6
]}

机构：

[1] Univ Halle Wittenberg, Biozentrum, D-06120 Halle, Germany

[2] Univ Leipzig, Dept Oral Maxillofacial & Facial Plast Surg, D-04103 Leipzig, Germany

[3] Univ Halle Wittenberg, Inst Chem, D-06120 Halle, Germany

[4] Univ Rey Juan Carlos, Dept Quim Inorgan & Analit, ESCET, Madrid 28933, Spain

[5] Max Planck Res Unit Enzymol Prot Folding, D-06120 Halle, Germany

[6] Griffith Univ, Griffith Hlth Inst, Griffith, Qld 4222, Australia

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2011年 / 105卷 / 02期

关键词：

Anticancer drugs; Cytotoxicity; Apoptosis; Caspase activity; Cell cycle; Gallium(III) complexes; GALLIUM(III) COMPLEXES; METAL-COMPLEXES; CANCER-CELLS; CISPLATIN; MECHANISMS; DRUGS; DERIVATIVES; INHIBITION; TITANOCENE; EXPRESSION;

D O I：

10.1016/j.jinorgbio.2010.10.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Organogallium(III) dinuclear (1-9) and tetranuclear (10) complexes present potential therapeutic agents for the treatment of various types of cancer. The antiproliferative activity of 1-10 was evaluated with cell lines of head and neck squamous cell carcinomas, e.g. HN (soft palate), Cal27, Cal33 (tongue) and FaDu (hypopharynx) cell lines. The activity of compound 8 is comparable with that of cisplatin on cell line Cal27 (IC50 4.6 mu M for both compounds). The mode of cell death induced, caspase activity and cell cycle analysis were evaluated for potential hit compounds 3, 5 and 8 Potential hit compounds 3, 5 and 8 were further evaluated for the mode of cell death, caspase activity and cell cycle analysis. Apoptosis induced by compounds 3,5 and 8 on Cal27 and FaDu cells was confirmed by DNA laddering, as well as acridine orange (AO) and ethidium bromide (EB) double staining. These compounds (3,5 and 8) induced caspase-independent apoptosis (within 4 h of action) in cell line Cal27. Extrinsic-mediated apoptosis associated with caspase 8 and 3 activation is the main mode of cytotoxicity induced on FaDu cells by compounds 3, 5 and 8. Cell cycle perturbations caused by these compounds are also observed. Our data suggest that compounds 3, 5 and 8 should be studied further for the treatment of head and neck cancer. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：164 / 170

页数：7

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