Plant regeneration from calli in giant reed (Arundo donax L.)

被引:15
|
作者
Takahashi, Wataru [1 ]
Takamizo, Tadashi [1 ]
Kobayashi, Makoto [1 ]
Ebina, Masumi [1 ]
机构
[1] Natl Inst Livestock & Grassland Sci, Forage Crop Biotechnol Res Team, Nasushiobara, Tochigi 3292793, Japan
关键词
Bioenergy crop; lignocellulosic biomass; propagation; tissue culture; transgenic plant; MULTIPLE-SHOOT FORMATION; SOMATIC EMBRYOGENESIS; ITALIAN RYEGRASS; APICAL MERISTEMS; IN-VITRO; GROWTH;
D O I
10.1111/j.1744-697X.2010.00198.x
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
We established a plant regeneration system using callus culture for giant reed (Arundo donax L.). An in vitro propagation system allowed year-round provision of explants by tissue culture. Calli were induced from axillary buds isolated from plants grown in vitro. Callus induction media supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA) influenced both callus induction frequency and later plant regeneration on hormone-free media. Media supplemented with 3 mg L-1 2,4-D appeared to harmonize callus formation and subsequent shoot formation. In contrast, despite a low callus formation frequency, calli induced with 0.25 mg L-1 BA alone showed the highest shoot formation frequency. Data suggest genotypic effects in tissue culture response as well. This study reports the first data regarding the tissue culture of giant reed.
引用
收藏
页码:224 / 229
页数:6
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