Evidence-based clinical outcome of oocyte slow cooling

被引:57
作者
Borini, A.
Bianchi, V.
Bonu, M. A.
Sciajno, R.
Sereni, E.
Cattoli, M.
Mazzone, S.
Trevisi, M. R.
Iadarola, I.
Distratis, V.
Nalon, M.
Coticchio, G.
机构
[1] Tecnobios Procreaz, I-40125 Bologna, Italy
[2] Casa Cura Abano Terme, Abano Terme, Italy
[3] Casa Cura Villa Serena, Citta S Angelo, Italy
[4] Casa Cura Le Betulle, Appiano Gentile, Italy
关键词
cryopreservation; cumulative pregnancies; oocytes; pregnancy rate; slow cooling;
D O I
10.1016/S1472-6483(10)60706-7
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
In the last few years, there has been a significant improvement in oocyte cryopreservation techniques. To investigate the clinical significance of oocyte freezing, an assessment of the cumulative pregnancy rate per started cycle derived from the use of fresh and frozen-thawed oocytes was performed. Between 2004 and 2006, 749 cycles were carried out, in which no more than three fresh oocytes were inseminated either by standard IVF or microinjection. Supernumerary mature oocytes were cryopreserved by slow cooling. Cryopreservation of fresh embryos was performed in rare cases to prevent the risk of ovarian hyperstimulation syndrome using a standard embryo freezing protocol. Fresh embryo transfer cycles totalled 680, 257 of which resulted in pregnancy. The pregnancy rates per patient and per transfer were 34.3% and 37.8% respectively. When frozen-thawed oocytes were used, following 660 thawing cycles, 590 embryo transfers were performed in 510 patients. Eighty-eight pregnancies were achieved with embryos from frozen oocytes, with a success rate of 17.2% per cycle. When fresh and frozen-thawed cycles were combined, the number of pregnancies was 355, giving a cumulative pregnancy rate of 47.4%. Oocyte cryopreservation can contribute considerably to the overall clinical success, ensuring a cumulative rate approaching that achievable with embryo storage.
引用
收藏
页码:175 / 181
页数:7
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