Evaluation of Sensitivity of Multiplex PCR for Detection of Mycobacterium tuberculosis and Pneumocystis jirovecii in Clinical Samples

被引：9

作者：

Boondireke, Sirirat ^{[2
]}

Mungthin, Mathirut ^{[1
]}

Tan-ariya, Peerapan ^{[2
]}

Boonyongsunchai, Petchara ^{[3
]}

Naaglor, Tawee ^{[1
]}

Wattanathum, Anan ^{[3
]}

Treewatchareekorn, Sompong ^{[4
]}

Leelayoova, Saovanee ^{[1
]}

机构：

[1] Phramongkutklao Coll Med, Dept Parasitol, Bangkok 10400, Thailand

[2] Mahidol Univ, Fac Sci, Dept Microbiol, Bangkok 10400, Thailand

[3] Phramongkutklao Hosp, Dept Med, Bangkok 10400, Thailand

[4] Royal Thai Army, Army Inst Pathol, Dept Microbiol, Bangkok 10400, Thailand

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 09期

关键词：

ACQUIRED-IMMUNODEFICIENCY-SYNDROME; CARINII-PNEUMONIA; HIV-INFECTION; AIDS PATIENTS; VIRUS; AMPLIFICATION; DNA;

D O I：

10.1128/JCM.00323-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex PCR assay for the simultaneous detection of Mycobacterium tuberculosis and Pneumocystis jirovecii was developed using IS6110-based detection for M. tuberculosis and mitochondrial large-subunit (mtLSU) rRNA gene detection for P. jirovecii. Ninety-five pulmonary blinded samples were examined using the developed multiplex PCR assay, and the results were compared with those obtained by the single nested PCRs targeting IS6110 for M. tuberculosis and mtLSU rRNA for P. jirovecii. Of the 95 pulmonary samples tested, the multiplex nested PCR developed here could detect 36 cases of M. tuberculosis infection, 35 cases of P. jirovecii infection, and 17 cases of M. tuberculosis and P. jirovecii coinfections. The sensitivities of the multiplex nested PCR in detecting M. tuberculosis and P. jirovecii were 92.1% and 81.4%, respectively, whereas the specificities in detecting M. tuberculosis and P. jirovecii were 98.2% and 100%, respectively.

引用

页码：3165 / 3168

页数：4

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