Effects of the Global Regulator CsrA on the BarA/UvrY Two-Component Signaling System

被引：46

作者：

Camacho, Martha I. ^{[1
]}

Alvarez, Adrian F. ^{[1
]}

Gonzalez Chavez, Ricardo ^{[1
]}

Romeo, Tony ^{[2
]}

Merino, Enrique ^{[3
]}

Georgellis, Dimitris ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Dept Mol Genet, Inst Fisiol Celular, Mexico City 04510, DF, Mexico

[2] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

[3] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Mol Microbiol, Cuernavaca 62191, Morelos, Mexico

来源：

JOURNAL OF BACTERIOLOGY | 2015年 / 197卷 / 05期

基金：

美国国家卫生研究院;

关键词：

RNA-BINDING PROTEIN; BARA SENSOR KINASE; ESCHERICHIA-COLI; MESSENGER-RNA; PSEUDOMONAS-AERUGINOSA; RESPONSE REGULATOR; ACETYL PHOSPHATE; FLHDC EXPRESSION; GENE-EXPRESSION; IDENTIFICATION;

D O I：

10.1128/JB.02325-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The hybrid sensor kinase BarA and its cognate response regulator UvrY, members of the two-component signal transduction family, activate transcription of CsrB and CsrC noncoding RNAs. These two small RNAs act by sequestering the RNA binding protein CsrA, which posttranscriptionally regulates translation and/or stability of its target mRNAs. Here, we provide evidence that CsrA positively affects, although indirectly, uvrY expression, at both the transcriptional and translational levels. We also demonstrate that CsrA is required for properly switching BarA from its phosphatase to its kinase activity. Thus, the existence of a feedback loop mechanism that involves the Csr and BarA/UvrY global regulatory systems is exposed.

引用

页码：983 / 991

页数：9

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