Label-free, real-time on-chip sensing of living cells via grating-coupled surface plasmon resonance

被引：25

作者：

Borile, Giulia ^{[1
,2
]}

Rossi, Stefano ^{[1
,2
]}

Filippi, Andrea ^{[1
,2
,3
]}

Gazzola, Enrico ^{[2
]}

Capaldo, Pietro ^{[2
,4
]}

Tregnago, Claudia ^{[5
]}

Pigazzi, Martina ^{[1
,5
]}

Romanato, Filippo ^{[1
,2
,4
,6
]}

机构：

[1] Fdn Inst Pediat Res Citta Speranza, Corso Stati Uniti 4, I-35127 Padua, Italy

[2] Univ Padua, Dept Phys & Astron G Galilei, Via Marzolo 8, I-35131 Padua, Italy

[3] Fdn Bruno Kessler, Via Sommar 18, I-38123 Trento, Italy

[4] CNR, INFM, TASC IOM Natl Lab, Area Sci Pk SS,14 Km 163-5, I-34012 Trieste, Italy

[5] Univ Padua, Haematol Oncol Clin & Lab, Women & Child Hlth Dept, Via Giustiniani 3, I-35128 Padua, Italy

[6] Lab Nanofabricat Nanodevices, Corso Stati Uniti 4, I-35127 Padua, Italy

来源：

BIOPHYSICAL CHEMISTRY | 2019年 / 254卷

关键词：

Surface plasmon resonance; Grating; Nanostructure; Live cells; ADHESION;

D O I：

10.1016/j.bpc.2019.106262

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of nanotechnologies to address biomedical questions is a key strategy for innovation in biomedical research. Among others, a key point consists in the availability of nanotechnologies for monitoring cellular processes in a real-time and label-free approach. Here, we focused on a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor exploiting phase interrogation. This sensor can be integrated in a microfluidic chamber that ensures cell viability and avoids cell stress. We report the calibration of the sensor response as a function of cell number and its application to monitor cell adhesion kinetics as well as cell response to an external stimulus. Our results show that GC-SPR sensors can offer a valuable alternative to prism-coupled or imaging SPR devices, amenable for microfluidic implementation.

引用

页数：6

共 27 条

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