First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia

被引：24

作者：

Alhamami, Tamara ^{[1
]}

Chowdhury, Piklu Roy ^{[2
]}

Gomes, Nancy ^{[1
]}

Carr, Mandi ^{[3
]}

Veltman, Tania ^{[1
]}

Khazandi, Manouchehr ^{[1
]}

Mollinger, Joanne ^{[4
]}

Deutscher, Ania T. ^{[5
]}

Turni, Conny ^{[6
]}

Mahdi, Layla ^{[7
]}

Venter, Henrietta ^{[7
]}

Abraham, Sam ^{[8
]}

Djordjevic, Steven P. ^{[2
]}

Trott, Darren J. ^{[1
]}

机构：

[1] Univ Adelaide, Australian Ctr Antimicrobial Resistance Ecol, Sch Anim & Vet Sci, Roseworthy Campus, Roseworthy, SA 5371, Australia

[2] Univ Technol Sydney, Ithree Inst, City Campus, Ultimo, NSW 2007, Australia

[3] Univ Adelaide, Sch Anim & Vet Sci, Dept Anim Hlth & Prod, Roseworthy, SA 5371, Australia

[4] Dept Agr & Fisheries, Biosecur Sci Lab, Coopers Plains, Qld 4108, Australia

[5] Elizabeth Macarthur Agr Inst, NSW Dept Primary Ind, Menangle, NSW 2568, Australia

[6] Univ Queensland, Queensland Alliance Agr & Food Innovat, Ctr Anim Sci, St Lucia, Qld 4072, Australia

[7] Univ South Australia, Clin & Hlth Sci, Adelaide, SA 5000, Australia

[8] Murdoch Univ, Antimicrobial Resistance & Infect Dis Lab, Murdoch, WA 6150, Australia

来源：

MICROORGANISMS | 2021年 / 9卷 / 06期

关键词：

bovine respiratory disease; antimicrobial susceptibility; Mannheimia haemolytica; Pasteurella multocida; BOVINE RESPIRATORY-DISEASE; ANTIMICROBIAL SUSCEPTIBILITY; CATTLE; PATHOGENS; GENES; TRACT; EPIDEMIOLOGY; BACTERIA; PREVALENCE; TILMICOSIN;

D O I：

10.3390/microorganisms9061322

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bovine respiratory disease (BRD) causes high morbidity and mortality in beef cattle worldwide. Antimicrobial resistance (AMR) monitoring of BRD pathogens is critical to promote appropriate antimicrobial stewardship in veterinary medicine for optimal treatment and control. Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014-2019) was determined for 19 antimicrobial agents. The M. haemolytica isolates were pan-susceptible to all tested agents apart from a single macrolide-resistant isolate (1/88; 1.1%) from New South Wales (NSW). Much higher frequencies of P. multocida isolates were resistant to tetracycline (18/140; 12.9%), tilmicosin (19/140; 13.6%), tulathromycin/gamithromycin (17/140; 12.1%), and ampicillin/penicillin (6/140; 4.6%). Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and bla(ROB-1) encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. These results confirm the first emergence of AMR in M. haemolytica and P. multocida from BRD cases in Australia, which should be closely monitored.

引用

页数：17

共 57 条

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