MULTICOMPONENT ONE-POT FACILE SYNTHESIS OF PYRIMIDINE DERIVATIVES UNDER MICROWAVE IRRADIATION TECHNIQUE AND STUDY OF THEIR ANTI-INFLAMMATORY ACTIVITY

Multicomponent one-pot synthetic protocol is applied for the efficient preparation of a series of pyrimidine derivative under microwave irradiation method. The synthetic process proceeds via Knoevenagel condensation initially with subsequent Michael addition reaction followed by cyclization of equimolar quantities of substituted bezaldehydes, ethylcyanoacetate and guanidine in the presence of ethanolic sodium hydroxide solution to produce corresponding pyrimidine derivatives. The reaction mixture was refluxed under microwave radiation for 7-12min at power level-2 which corresponds to 210watt. Microwave heating provides cleaner reaction condition with shorter reaction time and improved product yield as compared to conventional heating method. The structures of the newly synthesized compounds were elucidated by their FT-IR, H-1-NMR and LC-MS spectral data. The newly synthesized compounds were screened for their anti-inflammatory activity in-vitro by using membrane stabilization method (heat induced hemolytic technique). Some of the tested compounds were found to possess significant anti-inflammatory potential as compared to the standard drug (Diclofenac).