Enterovirus Infection Restricts Long Interspersed Element 1 Retrotransposition

被引：2
作者：
Li, Yan [1 ]
Shen, Siyu [1 ]
Guo, Haoran [1 ,2 ]
Zhang, Zhe [1 ]
Zhang, Lili [1 ]
Yang, Qingran [1 ]
Gao, Yanhang [3 ]
Niu, Junqi [3 ]
Wei, Wei [1 ,2 ]
机构：
[1] Jilin Univ, Hosp 1, Inst Virol & AIDS Res, Changchun, Peoples R China
[2] Jilin Univ, Hosp 1, Inst Translat Med, Key Lab Organ Regenerat & Transplantat,Minist Edu, Changchun, Peoples R China
[3] Jilin Univ, Hosp 1, Dept Hepatol, Changchun, Peoples R China
来源：
FRONTIERS IN MICROBIOLOGY | 2021年 / 12卷
基金：
中国国家自然科学基金;
关键词：
enterovirus; LINE-1; EV-D68; EV-A71; retrotransposon; 3C PROTEASE; L1; LINE-1; ACTIVATION; CLEAVAGE; RNA;
D O I：
10.3389/fmicb.2021.706241
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Long interspersed element 1 (LINE-1 or L1) is the only active autonomous retrotransposon in the human genome that can serve as an endogenous upstream activator of cytoplasmic nucleic acid sensing pathways to elicit an antiviral immune response. In this study, we investigated the influence of enteroviral infection on L1 mobility. The results showed that infection with different enteroviruses, both EV-D68 and EV-A71, blocked L1 transposition. We screened diverse viral accessory proteins for L1 activity and identified EV-D68 2A, 3A, 3C, and EV-A71 ORF2p proteins as viral L1 inhibitors. EV-D68 2A suppressed L1 mobility by expression suppression of L1 proteins. Viral proteins 3A and 3C restricted ORF2p-mediated L1 reverse transcription in isolated L1 ribonucleoproteins. The newly identified enteroviral protein ORF2p inhibited the expression of L1 ORF1p. Altogether, our findings shed light on the strict modulation of L1 retrotransposons during enterovirus replication.
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页数：12
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