Detection and risk assessment of diarrheagenic E-coli in recreational beaches of Brazil

被引：18
作者：
Rodrigues, Vanessa F. V. [1 ,2 ]
Rivera, Irma N. G. [1 ]
Lim, Keah-Ying [2 ]
Jiang, Sunny C. [2 ]
机构：
[1] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508000 Estado De Sao Paulo, SP, Brazil
[2] Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA 92697 USA
来源：
MARINE POLLUTION BULLETIN | 2016年 / 109卷 / 01期
基金：
美国国家科学基金会;
关键词：
Diarrheagenic E. coli; Recreational health; Quantitative microbial risk assessment; Brazilian beaches; WATER-QUALITY; VIRULENCE GENES; ILLNESS; INDICATORS; POLLUTION; TOXIN; IDENTIFICATION; CONTAMINATION; CALIFORNIA; DIVERSITY;
D O I：
10.1016/j.marpolbul.2016.06.007
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Marine beaches are important recreational and economic resources in Brazil, but the beaches' water quality is negatively impacted by the discharge of domestic sewage effluent. The occurrence of diarrheagenic Escherichia coil among the E. coli isolated from three Brazilian marine beaches was investigated. Multiplex and single step PCR were used to screen 99 E. coli isolates for ten target toxin genes. Six toxin genes, stx1, eae, estp, esth, astA, and bfpA, were identified in 1% to 35% of the isolates. A quantitative microbial risk assessment (QMRA) of human exposure to diarrheagenic E. coli during marine recreation was carried out. The results indicated that the diarrheagenic E. coli risk is well below the U.S. EPA's recommended daily recreational risk benchmark. However, the overall recreational health risk due to all pathogens in the water could be much higher and exceeded the U.S. EPA's benchmark. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：163 / 170
页数：8
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