Effect of freeze-drying on the mechanical, physical and morphological properties of glutaraldehyde-treated bovine pericardium: evaluation of freeze-dried treated bovine pericardium properties

被引:7
|
作者
Borgognoni, Camila F. [1 ]
Maizato, Marina J. S. [2 ]
Leirner, Adolfo A. [2 ]
Polakiewicz, Bronislaw [1 ]
Beppu, Marisa M. [3 ]
Higa, Olga Z. [4 ]
Pitombo, Ronaldo N. M. [1 ]
机构
[1] Univ Sao Paulo, Pharmaceut Sci Coll, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo, Brazil
[2] Univ Sao Paulo, Sch Med, Heart Inst InCor, BR-05508000 Sao Paulo, Brazil
[3] Univ Estadual Campinas, Chem Engn Sch, Campinas, SP, Brazil
[4] Inst Energet & Nucl Res, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Bovine pericardium; Glutaraldehyde; Freeze-drying; LYOPHILIZATION; BIOMATERIALS; STRENGTH; BEHAVIOR; TISSUE; VALVES;
D O I
10.5301/JABB.2010.6104
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Purpose: Biomaterials have been widely used in the field of regenerative medicine. Bovine pericardium tissue has been successfully used as a bioprosthetic material in manufacturing heart valves, but studies concerning the tissue are ongoing in order to improve its storage, preservation and transportation. This article provides an overview of the characteristics of bovine pericardium tissue chemically treated after the freeze-drying process. These characteristics are essential to evaluate the changes or damage to the tissue during the process. Methods: The mechanical properties of the tissue were analyzed by three different methods due to its anisotropic characteristics. The physical properties were analyzed by a colorimetric method, while the morphological properties were evaluated by scanning electron microscopy (SEM). Results: The freeze-dried bovine pericardium showed no significant change in its mechanical properties. There was no significant change in the elasticity of the tissue (p > 0.05) and no color change. In addition, SEM analysis showed that the freeze-dried samples did not suffer structural collapse. Conclusions: It was concluded that glutaraldehyde-treated bovine pericardium tissue showed no significant change in its properties after the freeze-drying process.
引用
收藏
页码:186 / 190
页数:5
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