Modified Lactic Acid Bacteria Detect and Inhibit Multiresistant Enterococci

被引：81
作者：
Borrero, Juan [1 ]
Chen, Yuqing [2 ]
Dunny, Gary M. [2 ]
Kaznessis, Yiannis N. [1 ]
机构：
[1] Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Microbiol, Minneapolis, MN 55455 USA
来源：
ACS SYNTHETIC BIOLOGY | 2015年 / 4卷 / 03期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
lactic acid bacteria; Lactococcus lactis; Enterococcus faecalis; drug delivery vector; recombinant plasmids; SEC-DEPENDENT BACTERIOCIN; GENE-EXPRESSION; THERAPEUTIC MOLECULES; LACTOCOCCUS-LACTIS; PHEROMONE; FAECIUM; PROTEIN; SYSTEM; LACTOBACILLI; CONJUGATION;
D O I：
10.1021/sb500090b
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We designed Lactococcus lactis to detect Enterococcus faecalis. Upon detection, L. lactis produce and secrete antienterococcal peptides. The peptides inhibit enterococcal growth and reduce viability of enterococci in the vicinity of L. lactis. The enterococcal sex pheromone cCF10 serves as the signal for detection. Expression vectors derived from pCF10, a cCF10-responsive E. faecalis sex-pheromone conjugative plasmid, were engineered in L. lactis for the detection system. Recombinant host strains were engineered to express genes for three bacteriocins, enterocin A, hiracin JM79 and enterocin P, each with potent antimicrobial activity against E. faecalis. Sensitive detection and specific inhibition occur both in agar and liquid media. The engineered L. lactis also inhibited growth of multidrug-resistant E. faecium strains, when induced by cCF10. The presented vectors and strains can be components of a toolbox for the development of alternative antibiotic technologies targeting enterococci at the site of infection.
引用
收藏
页码：299 / 306
页数：8
相关论文
共 39 条
[1]   Identification of Bacteriocin Genes in Enterococci Isolated from Game Animals and Saltwater Fish [J].
Almeida, Tereza ;
Brandao, Andreia ;
Munoz-Atienza, Estefania ;
Goncalves, Alexandre ;
Torres, Carmen ;
Igrejas, Gilberto ;
Hernandez, Pablo E. ;
Herranz, Carmen ;
Cintas, Luis M. ;
Poeta, Patricia .
JOURNAL OF FOOD PROTECTION, 2011, 74 (08) :1252-1260
[2]  
[Anonymous], 1989, Molecular Cloning
[3]   ccfA, the genetic determinant for the cCF10 peptide pheromone in Enterococcus faecalis OG1RF [J].
Antiporta, MH ;
Dunny, GM .
JOURNAL OF BACTERIOLOGY, 2002, 184 (04) :1155-1162
[4]   The rise of the Enterococcus: beyond vancomycin resistance [J].
Arias, Cesar A. ;
Murray, Barbara E. .
NATURE REVIEWS MICROBIOLOGY, 2012, 10 (04) :266-278
[5]   Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].
Aymerich, T ;
Holo, H ;
Havarstein, LS ;
Hugas, M ;
Garriga, M ;
Nes, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1676-1682
[6]   Development of a Stress-Inducible Controlled Expression (SICE) system in Lactococcus lactis for the production and delivery of therapeutic molecules at mucosal surfaces [J].
Benbouziane, Bouasria ;
Ribelles, Pedro ;
Aubry, Camille ;
Martin, Rebeca ;
Kharrat, Pascale ;
Riazi, Ali ;
Langella, Philippe ;
Bermudez-Humaran, Luis G. .
JOURNAL OF BIOTECHNOLOGY, 2013, 168 (02) :120-129
[7]   Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines [J].
Bermudez-Humaran, Luis G. ;
Kharrat, Pascale ;
Chatel, Jean-Marc ;
Langella, Philippe .
MICROBIAL CELL FACTORIES, 2011, 10
[8]   Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria [J].
Borrero, Juan ;
Jimenez, Juan J. ;
Gutiez, Loreto ;
Herranz, Carmen ;
Cintas, Luis M. ;
Hernandez, Pablo E. .
JOURNAL OF BIOTECHNOLOGY, 2011, 156 (01) :76-86
[9]   Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expression of enterococcal bacteriocins in Lactococcus lactis [J].
Borrero, Juan ;
Jimenez, Juan J. ;
Gutiez, Loreto ;
Herranz, Carmen ;
Cintas, Luis M. ;
Hernandez, Pablo E. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 89 (01) :131-143
[10]   Tweaking biological switches through a better understanding of bistability behavior [J].
Chatterjee, Anushree ;
Kaznessis, Yiannis N. ;
Hu, Wei-Shou .
CURRENT OPINION IN BIOTECHNOLOGY, 2008, 19 (05) :475-481
1234