Bacillus thuringiensis cry1Ab gene confers resistance to potato against Helicoverpa armigera (Hubner)

被引：14

作者：

Chakrabarti, SK ^{[1
]}

Mandaokar, AD

Shukla, A

Pattanayak, D

Naik, PS

Sharma, RP

Kumar, PA

机构：

[1] Cent Potato Res Inst, Shimla 171001, Himachal Prades, India

[2] Indian Agr Res Inst, Natl Res Ctr Plant Biotechnol, New Delhi 1100012, India

来源：

POTATO RESEARCH | 2000年 / 43卷 / 02期

关键词：

transgenic plants; Solanum tuberosum L;

D O I：

10.1007/BF02357955

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal crystal protein (Cry1Ab) of Bacillus thuringiensis (Bt) has been introduced into five genotypes of potato using Agrobacterium tumefaciens. Southern analysis of DNA from transgenic plants confirmed the integration and copy number of the transgene. Double-antibody quantitative sandwich ELISA analysis demonstrated high levels of Cry1Ab protein expression in transgenic plants. Insect bioassays on the leaves of transgenic plants showed considerable protection against the larvae of H. armigera in terms of leaf area consumed and larval weight reduction.

引用

页码：143 / 152

页数：10

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