Purification and partial characterization of a thrombin-like enzyme, balterobin, from the venom of Bothrops alternatus

被引：33

作者：

Smolka, MB ^{[1
]}

Marangoni, S ^{[1
]}

Oliveira, B ^{[1
]}

Novello, JC ^{[1
]}

机构：

[1] Univ Estadual Campinas, Dept Bioquim, BR-13081970 Campinas, SP, Brazil

来源：

TOXICON | 1998年 / 36卷 / 07期

关键词：

D O I：

10.1016/S0041-0101(98)80008-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A thrombin-like enzyme, balterobin, was purified from the venom of Bothrops alternatus. The purification steps included Sephadex G-75, heparin-sepharose and reverse phase HPLC C-IX column. Balterobin showed an apparent molecular weight of 30 000 in non-reduced conditions and displays a specific coagulant activity of 32.8 NIH units/mg over bovine fibrinogen. It also exhibits arginine amidase activity on DL-BAPNA. Like thrombin-like enzymes from other snakes, balterobin possesses valine as N-terminal residue, and is inhibited by PMSF. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1059 / 1063

页数：5

共 14 条

[1] CHARACTERIZATION OF COAGULANT ACTIVITY OF SOME SNAKE-VENOMS [J].

DENSON, KWE ;

ALMAGRO, D ;

BISHOP, RC ;

RUSSELL, FE .

TOXICON, 1972, 10 (06) :557-&

[2] PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].

ERLANGER, BF ;

COHEN, W ;

KOKOWSKY, N .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&

[3] CHARACTERIZATION OF CERASTOBIN, A THROMBIN-LIKE ENZYME FROM THE VENOM OF CERASTES-VIPERA (SAHARA SAND VIPER) [J].

FARID, TM ;

TU, AT ;

ELASMAR, MF .

BIOCHEMISTRY, 1989, 28 (01) :371-377

[4] STUDIES ON COAGULANT ENZYME FROM AGKISTRODON-RHODOSTOMA VENOM - ISOLATION AND SOME PROPERTIES OF ENZYME [J].

HATTON, MWC .

BIOCHEMICAL JOURNAL, 1973, 131 (04) :799-807

[5]

ITOH N, 1988, J BIOL CHEM, V263, P7628

[6] HEMOSTATIC CHANGES CAUSED BY THE VENOMS OF SOUTH-AMERICAN SNAKES [J].

KAMIGUTI, AS ;

CARDOSO, JLC .

TOXICON, 1989, 27 (09) :955-963

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8] RAPID METHOD FOR PURIFICATION OF BOVINE THROMBIN AND INHIBITION OF PURIFIED ENZYME WITH PHENYLMETHYLSULFONYL FLUORIDE [J].

LUNDBLAD, RL .

BIOCHEMISTRY, 1971, 10 (13) :2501-&

[9]

MARKLAND FS, 1971, J BIOL CHEM, V246, P6460

[10]

MATSUDAIRA P, 1987, J BIOL CHEM, V262, P10035

← 1 2 →