Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants

被引:1
作者
Paweska, Janusz T. [1 ,2 ,3 ]
Jansen van Vuren, Petrus [4 ]
Msimang, Veerle [1 ]
Lo, Modu Moustapha [5 ]
Thiongane, Yaya [5 ]
Mulumba-Mfumu, Leopold K. [6 ,7 ]
Mansoor, Alqadasi [8 ,9 ]
Fafetine, Jose M. [10 ]
Magona, Joseph W. [11 ,12 ]
Boussini, Hiver [13 ,14 ]
Bazanow, Barbara [15 ]
Wilson, William C. [16 ]
Pepin, Michel [17 ,18 ]
Unger, Hermann [19 ]
Viljoen, Gerrit [19 ]
机构
[1] Natl Inst Communicable Dis, Natl Hlth Lab Serv, Ctr Emerging Zoonot & Parasit Dis, ZA-2131 Johannesburg, South Africa
[2] Univ Pretoria, Fac Hlth Sci, Dept Med Virol, Ctr Viral Zoonoses, ZA-0001 Pretoria, South Africa
[3] Univ Witwatersrand, Sch Pathol, Fac Hlth Sci, ZA-2050 Johannesburg, South Africa
[4] CSIRO Hlth & Biosecur, Australian Ctr Dis Preparedness, Geelong, Vic 3220, Australia
[5] Lab Natl Elevage & Rech Vet, Route Front Terre, Dakar 2057, Senegal
[6] Minist Agr, Kinshasa 7948, DEM REP CONGO
[7] Univ Kinshasa, Dept Clin Sci, Fac Vet Med, POB 127, Kinshasa Xi, DEM REP CONGO
[8] Minist Agr & Irrigat, Cent Vet Lab, Gen Directorate Anim Hlth & Vet Quarantine, Sanaa 31220, Yemen
[9] Food & Agr Org Off, Sanaa 31220, Yemen
[10] Eduardo Mondlane Univ, Vet Fac, Maputo 1103, Mozambique
[11] Natl Livestock Resources Res Inst, POB 96, Tororo, Uganda
[12] Food & Agr Org, POB 54, Gaborone, Botswana
[13] Minist Ressources Anim, Direct Generale Serv Vet, 09 BP 907, Ouagadougou, Burkina Faso
[14] African Union Interafrican Bur Anim Resources, POB 30786-00100, Nairobi, Kenya
[15] Univ Environm & Life Sci, Dept Pathol, Fac Vet Sci, PL-50375 Wroclaw, Poland
[16] ARS, USDA, Foreign Arthropod Borne Anim Dis Res Unit, Natl Bio & Agrodef Facil, Manhattan, KS 66502 USA
[17] Agence Francaise Securite Sanit Aliments, F-69364 Lyon, France
[18] Campus Vet Lyon, VetAgro Sup, F-69364 Lyon, France
[19] IAEA, Joint FAO IAEA Ctr Nucl Techn Food & Agr, A-1400 Vienna, Austria
来源
VIRUSES-BASEL | 2021年 / 13卷 / 08期
关键词
Rift Valley fever virus; enzyme-linked immunosorbent assay; recombinant nucleocapsid; IgG antibody; domestic ruminants; validation; diagnostic accuracy; LINKED-IMMUNOSORBENT-ASSAY; MARBURG VIRUSES; RAPID DETECTION; CAPTURE ELISA; N-PROTEIN; DIAGNOSIS; SANDWICH; PCR; HUMANS; TESTS;
D O I
10.3390/v13081651
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures.
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