Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants

被引：2

作者：

Paweska, Janusz T. ^{[1
,2
,3
]}

Jansen van Vuren, Petrus ^{[4
]}

Msimang, Veerle ^{[1
]}

Lo, Modu Moustapha ^{[5
]}

Thiongane, Yaya ^{[5
]}

Mulumba-Mfumu, Leopold K. ^{[6
,7
]}

Mansoor, Alqadasi ^{[8
,9
]}

Fafetine, Jose M. ^{[10
]}

Magona, Joseph W. ^{[11
,12
]}

Boussini, Hiver ^{[13
,14
]}

Bazanow, Barbara ^{[15
]}

Wilson, William C. ^{[16
]}

Pepin, Michel ^{[17
,18
]}

Unger, Hermann ^{[19
]}

Viljoen, Gerrit ^{[19
]}

机构：

[1] Natl Inst Communicable Dis, Natl Hlth Lab Serv, Ctr Emerging Zoonot & Parasit Dis, ZA-2131 Johannesburg, South Africa

[2] Univ Pretoria, Fac Hlth Sci, Dept Med Virol, Ctr Viral Zoonoses, ZA-0001 Pretoria, South Africa

[3] Univ Witwatersrand, Sch Pathol, Fac Hlth Sci, ZA-2050 Johannesburg, South Africa

[4] CSIRO Hlth & Biosecur, Australian Ctr Dis Preparedness, Geelong, Vic 3220, Australia

[5] Lab Natl Elevage & Rech Vet, Route Front Terre, Dakar 2057, Senegal

[6] Minist Agr, Kinshasa 7948, DEM REP CONGO

[7] Univ Kinshasa, Dept Clin Sci, Fac Vet Med, POB 127, Kinshasa Xi, DEM REP CONGO

[8] Minist Agr & Irrigat, Cent Vet Lab, Gen Directorate Anim Hlth & Vet Quarantine, Sanaa 31220, Yemen

[9] Food & Agr Org Off, Sanaa 31220, Yemen

[10] Eduardo Mondlane Univ, Vet Fac, Maputo 1103, Mozambique

[11] Natl Livestock Resources Res Inst, POB 96, Tororo, Uganda

[12] Food & Agr Org, POB 54, Gaborone, Botswana

[13] Minist Ressources Anim, Direct Generale Serv Vet, 09 BP 907, Ouagadougou, Burkina Faso

[14] African Union Interafrican Bur Anim Resources, POB 30786-00100, Nairobi, Kenya

[15] Univ Environm & Life Sci, Dept Pathol, Fac Vet Sci, PL-50375 Wroclaw, Poland

[16] ARS, USDA, Foreign Arthropod Borne Anim Dis Res Unit, Natl Bio & Agrodef Facil, Manhattan, KS 66502 USA

[17] Agence Francaise Securite Sanit Aliments, F-69364 Lyon, France

[18] Campus Vet Lyon, VetAgro Sup, F-69364 Lyon, France

[19] IAEA, Joint FAO IAEA Ctr Nucl Techn Food & Agr, A-1400 Vienna, Austria

来源：

VIRUSES-BASEL | 2021年 / 13卷 / 08期

关键词：

Rift Valley fever virus; enzyme-linked immunosorbent assay; recombinant nucleocapsid; IgG antibody; domestic ruminants; validation; diagnostic accuracy; LINKED-IMMUNOSORBENT-ASSAY; MARBURG VIRUSES; RAPID DETECTION; CAPTURE ELISA; N-PROTEIN; DIAGNOSIS; SANDWICH; PCR; HUMANS; TESTS;

D O I：

10.3390/v13081651

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures.

引用

页数：22

共 79 条

[11] Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, Dengue virus, and Yellow fever virus by real-time reverse transcription-PCR [J].