共 79 条
Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants
被引:1
作者:
Paweska, Janusz T.
[1
,2
,3
]
Jansen van Vuren, Petrus
[4
]
Msimang, Veerle
[1
]
Lo, Modu Moustapha
[5
]
Thiongane, Yaya
[5
]
Mulumba-Mfumu, Leopold K.
[6
,7
]
Mansoor, Alqadasi
[8
,9
]
Fafetine, Jose M.
[10
]
Magona, Joseph W.
[11
,12
]
Boussini, Hiver
[13
,14
]
Bazanow, Barbara
[15
]
Wilson, William C.
[16
]
Pepin, Michel
[17
,18
]
Unger, Hermann
[19
]
Viljoen, Gerrit
[19
]
机构:
[1] Natl Inst Communicable Dis, Natl Hlth Lab Serv, Ctr Emerging Zoonot & Parasit Dis, ZA-2131 Johannesburg, South Africa
[2] Univ Pretoria, Fac Hlth Sci, Dept Med Virol, Ctr Viral Zoonoses, ZA-0001 Pretoria, South Africa
[3] Univ Witwatersrand, Sch Pathol, Fac Hlth Sci, ZA-2050 Johannesburg, South Africa
[4] CSIRO Hlth & Biosecur, Australian Ctr Dis Preparedness, Geelong, Vic 3220, Australia
[5] Lab Natl Elevage & Rech Vet, Route Front Terre, Dakar 2057, Senegal
[6] Minist Agr, Kinshasa 7948, DEM REP CONGO
[7] Univ Kinshasa, Dept Clin Sci, Fac Vet Med, POB 127, Kinshasa Xi, DEM REP CONGO
[8] Minist Agr & Irrigat, Cent Vet Lab, Gen Directorate Anim Hlth & Vet Quarantine, Sanaa 31220, Yemen
[9] Food & Agr Org Off, Sanaa 31220, Yemen
[10] Eduardo Mondlane Univ, Vet Fac, Maputo 1103, Mozambique
[11] Natl Livestock Resources Res Inst, POB 96, Tororo, Uganda
[12] Food & Agr Org, POB 54, Gaborone, Botswana
[13] Minist Ressources Anim, Direct Generale Serv Vet, 09 BP 907, Ouagadougou, Burkina Faso
[14] African Union Interafrican Bur Anim Resources, POB 30786-00100, Nairobi, Kenya
[15] Univ Environm & Life Sci, Dept Pathol, Fac Vet Sci, PL-50375 Wroclaw, Poland
[16] ARS, USDA, Foreign Arthropod Borne Anim Dis Res Unit, Natl Bio & Agrodef Facil, Manhattan, KS 66502 USA
[17] Agence Francaise Securite Sanit Aliments, F-69364 Lyon, France
[18] Campus Vet Lyon, VetAgro Sup, F-69364 Lyon, France
[19] IAEA, Joint FAO IAEA Ctr Nucl Techn Food & Agr, A-1400 Vienna, Austria
来源:
VIRUSES-BASEL
|
2021年
/
13卷
/
08期
关键词:
Rift Valley fever virus;
enzyme-linked immunosorbent assay;
recombinant nucleocapsid;
IgG antibody;
domestic ruminants;
validation;
diagnostic accuracy;
LINKED-IMMUNOSORBENT-ASSAY;
MARBURG VIRUSES;
RAPID DETECTION;
CAPTURE ELISA;
N-PROTEIN;
DIAGNOSIS;
SANDWICH;
PCR;
HUMANS;
TESTS;
D O I:
10.3390/v13081651
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures.
引用
收藏
页数:22
相关论文