INFLUENCE OF CULTURE CONDITIONS ON LIGNINOLYTIC ENZYMES PRODUCTION FROM Ganoderma lucidum USING AGRO-INDUSTRIAL RESIDUES

被引：0

作者：

Nisa, Fasiha Tun ^{[1
]}

Asgher, Muhammad ^{[2
]}

Anwar, Zahid ^{[1
]}

Afroz, Amber ^{[3
]}

Zeeshan, Nadia ^{[3
]}

Rashid, Umer ^{[3
]}

Irshad, Muhammad ^{[1
]}

Mehmood, Sajid ^{[1
]}

Sughra, Kalsoom ^{[3
]}

机构：

[1] NSMC Univ Gujrat, Dept Biochem, Gujrat, Pakistan

[2] Univ Agr Faisalabad, Dept Chem & Biochem, Faisalabad, Pakistan

[3] Univ Gujrat, Dept Biochem & Mol Biol, Gujrat, Pakistan

来源：

FRESENIUS ENVIRONMENTAL BULLETIN | 2014年 / 23卷 / 12C期

关键词：

G; lucidum; ligninolytic enzymes; process optimization; still culture; white rot fungi; TRAMETES-VERSICOLOR IBL-04; SOLID-STATE FERMENTATION; WHITE-ROT FUNGI; NITROGEN-SOURCES; LACCASE; DECOLORIZATION; OPTIMIZATION; EFFLUENTS; CORNCOBS; CARBON;

D O I：

暂无

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The present study was carried out to investigate the ligninolytic enzymes production potential of G. lucidum using different agro-industrial residues and their by-products. The enzyme profile of G. lucidum showed highest activities of manganese peroxidase (MnP) after 4th day of incubation on peanut shells followed by lignin peroxidase (LiP) and laccase after 6th and 7th day of incubation on orange peel and wheat straw, respectively. The enzyme with maximum activity (MnP) was optimized, therefore, through process optimization the production of MnP was substantially enhanced up to 2160 +/- 212 U/mL, when fermentation medium of peanut shells inoculated with 5 mL spore suspension of G. lucidum. Other optimum conditions were: pH, 5; moisture level, 70%; temperature, 40 degrees C; carbon source, glucose (1%); nitrogen supplement, yeast extract (0.2%), and modulator, MnSO4.

引用

页码：3442 / 3448

页数：7

共 21 条

[1] Laccase production by some white rot fungi under different nutritional conditions [J].