The fate of Mn2+ ions inside Saccharomyces cerevisiae cells seen by electron paramagnetic resonance

被引:9
作者
Farcasanu, IC
Ohta, N
Miyakawa, T
机构
[1] HIROSHIMA UNIV, FAC ENGN, DEPT FERMENTAT TECHNOL, HIGASHIHIROSHIMA 739, JAPAN
[2] HIROSHIMA UNIV, FAC ENGN, DEPT APPL PHYS & CHEM, HIGASHIHIROSHIMA 739, JAPAN
关键词
Saccharomyces cerevisiae; ion transport; manganese; EPR;
D O I
10.1271/bbb.60.468
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The fate of Mn2+ inside Saccharomyces cerevisiae cells was monitored during import and export processes, using information from electron paramagnetic resonance (EPR) spectroscopy analysis. EPR spectra showed that when entering the cell in high number, manganese ions immediately precipitated. If recorded under conditions that favored manganese efflux, EPR spectra indicated that only osmotically free ions were exported and that bound manganese had to turn into the soluble form before being able to leave the cell.
引用
收藏
页码:468 / 471
页数:4
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