GC-MS Method for Quantification and Pharmacokinetic Study of Four Volatile Compounds in Rat Plasma after Oral Administration of Commiphora myrrh (Nees) Engl. Resin and In Vitro Cytotoxic Evaluation

A rapid, simple, and sensitive gas chromatography-tandem mass spectrometry (GC-MS) method was established and validated for simultaneous determination of four volatile compounds, namely curzerene, methoxyfuranodiene, beta-elemene, and alpha-pinene in rat plasma samples after oral administration of the resin extract of Commiphora myrrh using limonene as an internal standard (IS). Liquid-liquid extraction using hexane and ethyl acetate (1:1) mixture as an extracting agent was used for the samples extraction procedure. The GC-MS system was operated under selective ion monitoring (SIM) mode using Perkin Elmer Elite 5MS column (30 m x 0.25 mm x 0.25 mu m film thickness). Specificity, linearity, precision, accuracy, extraction recovery, and stability were used to validate the developed method. The assay showed good linearity (r(2) >= 0.998), and the lowest limits of quantification (LLOQ) were 3.97-21.38 ng/mL for the four analytes. This assay was successfully applied to pharmacokinetic studies of the four volatile compounds in rat plasma. The antiproliferative activity of these volatile compounds was evaluated against lung carcinoma (A549) and colon (LoVo) cell lines, were each compound caused variable inhibition on cells proliferation and methoxyfuranodiene exerted the strong antiproliferative activity against both cell line according to IC50 values.