Enantiomeric separation by capillary electrochromatography using monolithic capillaries with sol-gel-glued cyclodextrin-modified silica particles

被引：41

作者：

Wistuba, D ^{[1
]}

Banspach, L ^{[1
]}

Schurig, V ^{[1
]}

机构：

[1] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany

来源：

ELECTROPHORESIS | 2005年 / 26卷 / 10期

关键词：

capillary electrochromatography; enantiomeric separation; monolith; permethyl-beta-cyclodextrin;

D O I：

10.1002/elps.200410251

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

By an on-column sol-gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl-beta-cyclodextrin-silica (Chira-Dex-silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kv) and pressure (300 bar) and possesses a high efficiency (up to 100 000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure-supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors.

引用

页码：2019 / 2026

页数：8

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