Enantiomeric separation by capillary electrochromatography using monolithic capillaries with sol-gel-glued cyclodextrin-modified silica particles

被引：41
|
作者：
Wistuba, D [1 ]
Banspach, L [1 ]
Schurig, V [1 ]
机构：
[1] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany
来源：
ELECTROPHORESIS | 2005年 / 26卷 / 10期
关键词：
capillary electrochromatography; enantiomeric separation; monolith; permethyl-beta-cyclodextrin;
D O I：
10.1002/elps.200410251
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
By an on-column sol-gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl-beta-cyclodextrin-silica (Chira-Dex-silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kv) and pressure (300 bar) and possesses a high efficiency (up to 100 000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure-supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors.
引用
收藏
页码：2019 / 2026
页数：8
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