The adjuvant effect of Myrtle, Myrtus communis, extract on hematological, Immuno-physiological, antioxidant responses, and tissue histomorphology of gill and liver in juvenile Siberian sturgeon, Acipenser baerii

This study aimed to evaluate the effect of Myrtle, Myrtus communis, extract on hematological, immuno-physiological, antioxidant responses, bactericidal activity, and tissue histomorphology of gill and liver in juvenile Siberian sturgeon, Acipenser baerii. Siberian sturgeon were exposed to 4 doses of myrtle extract including 25% (67.4 mg/L; M-25), 50% (134.9 mg/L; M-50), 75% (202.0 mg/L; M-75), and 100% (269.8 mg/L; M-100) of the maximum allowable concentration and a control treatment (without exposure myrtle extract). Hemoglobin and red blood cell values were significantly increased in fish exposed to the myrtle extracts (p<0.05). The white blood cell was lower in M-25 and M-75, while the highest value was found in M-100 treatment (p<0.05). Myrtle extract did not affect the lymphocyte value in the course of exposure (p>0.05). The highest albumin and total protein levels were observed in M-25 and M-50 groups. The highest values of lysozyme and total immunoglobulin (Ig) activities were observed in M-50, M-75 and M-25, M-50, respectively (p<0.05). Superoxide dismutase and catalase activities of those fish exposed to M-50 and M-75 were significantly higher than the control and M-100 groups (p<0.05). The lowest glutathione peroxidase value was observed in the control group compared to the others (p<0.05). The severe changes such as adhesion and curling of gill lamella discern were observed in fish exposed to different levels of myrtle extracts. Moreover, in the control group, severe hepatocyte destruction was accompanied by nucleus pyknosis, but the severity of atrophy was observed in M-75 and M-100 treatments. Overall, the results suggested that myrtle in the range of 67.4-202 mg/L could be applied as a stimulant agent to Siberian sturgeon aquaculture.