Second GHEP-ISFG exercise for DVI: "DNA-led" victims' identification in a simulated air crash

被引：6

作者：

Vullo, Carlos M. ^{[1
]}

Catelli, Laura ^{[1
]}

Ibarra Rodriguez, Adriana A. ^{[2
]}

Papaioannou, Aikaterini ^{[3
]}

Carlos Alvarez Merino, J. ^{[4
]}

Lopez-Parra, A. M. ^{[5
]}

Gaviria, Anibal ^{[6
]}

Baeza-Richer, Carlos ^{[5
]}

Romanini, Carola ^{[1
]}

Gonzalez-Moya, Esperanza ^{[7
]}

Casals, Ferran ^{[8
]}

Calafell, Francesc ^{[8
]}

Berardi, Gabriela ^{[12
]}

Carlo Iannacone, Gian ^{[10
]}

Vicuna Giraldo, Gloria C. ^{[11
]}

Zorba, Gulbanu K. ^{[3
]}

Boschi, Ilaria ^{[12
]}

Valdivia Olarte, Jane ^{[10
]}

Ruiz Gomez, Juan E. ^{[11
]}

Pablo Acierno, Juan ^{[1
]}

Lopez Soto, Manuel ^{[7
]}

Velazquez Miranda, Manuel ^{[13
]}

Garcia King, Marco D. ^{[14
]}

Alessandra Marrucci, Maria ^{[12
]}

Porto, Maria J. ^{[15
]}

Herrera Pinero, Mariana ^{[16
]}

Aler, Mercedes ^{[13
]}

Stephenson Ojea, Mishel M. ^{[14
]}

Cobos Navarrete, Santiago ^{[6
]}

Toscanini, Ulises ^{[9
]}

Saragoni, Victor G. ^{[17
]}

Bozzo, Walter ^{[16
]}

Posada Posada, Yeny C. ^{[2
]}

Bajunovic, Zlatan ^{[20
]}

Prieto Solla, Lourdes ^{[18
,19
]}

Parsons, Thomas ^{[20
]}

机构：

[1] Argentine Forens Anthropol Team EAAF, Cordoba, Argentina

[2] Univ Antioquia, Lab IdentiGEN, Medellin, Colombia

[3] Comm Missing Persons Cyprus CMP Anthropol Lab, Nicosia, Cyprus

[4] Univ Granada, Fac Med, Lab Identificac Genet, Granada, Spain

[5] Dept Toxicol & Hlth Legislat, Lab Forens & Populat Genet, Madrid, Spain

[6] Lab Genet Mol Cruz Vital Cruz Roja Ecuatoriana, Quito, Ecuador

[7] Inst Nacl Toxicol & Ciencias Forenses INTCF, Madrid, Spain

[8] Inst Biol Evolut CSIC UPF, Barcelona, Spain

[9] PRICAI Fdn Favaloro, Caba, Argentina

[10] Inst Med Legal, Lab Biol Mol & Genet, Lima, Peru

[11] Grp Genet Forense Direcc Reg Bogota, Bogota, Colombia

[12] Policlin Gemelli, Ist Sanita Pubbl Med Legale, Rome, Italy

[13] Inst Med Legal & Ciencias Forenses, Valencia, Spain

[14] Fdn Antropol Forense Guatemala FAFG, Guatemala City, Guatemala

[15] Inst Nacl Med Legal & Ciencias Forenses, Coimbra, Portugal

[16] Banco Nacl Datos Genet, Caba, Argentina

[17] Serv Med Legal, Unidad Genet Forense, Santiago, Chile

[18] USC, Grp Med Xenom, Inst Ciencias Forenses, Santiago De Compostela, Spain

[19] Comisaria Gen Policia Cient, Madrid, Spain

[20] Int Commiss Missing Persons ICMP, New York, NY USA

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2021年 / 53卷

关键词：

Disaster victim identification; MPI; Database comparison; DVI; Missing persons identification; CONTROL REGION VARIATION; COMMISSION;

D O I：

10.1016/j.fsigen.2021.102527

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Spanish and Portuguese-Speaking Working Group of the International Society for Forensic Genetics (GHEPISFG) has organized a second collaborative exercise on a simulated case of Disaster Victim Identification (DVI), with the participation of eighteen laboratories. The exercise focused on the analysis of a simulated plane crash case of medium-size resulting in 66 victims with varying degrees of fragmentation of the bodies (with commingled remains). As an additional difficulty, this second exercise included 21 related victims belonging to 6 families among the 66 missings to be identified. A total number of 228 post-mortem samples were represented with aSTR and mtDNA profiles, with a proportion of partial aSTR profiles simulating charred remains. To perform the exercise, participants were provided with aSTR and mtDNA data of 51 reference pedigrees -some of which deficient-including 128 donors for identification purposes. The exercise consisted firstly in the comparison of the post-mortem genetic profiles in order to re-associate fragmented remains to the same individual and secondly in the identification of the re-associated remains by comparing aSTR and mtDNA profiles with reference pedigrees using pre-established thresholds to report a positive identification. Regarding the results of the post-mortem samples re-associations, only a small number of discrepancies among participants were detected, all of which were from just a few labs. However, in the identification process by kinship analysis with family references, there were more discrepancies in comparison to the correct results. The identification results of single victims yielded fewer problems than the identification of multiple related victims within the same family groups. Several reasons for the discrepant results were detected: a) the identity/non-identity hypotheses were sometimes wrongly expressed in the likelihood ratio calculations, b) some laboratories failed to use all family references to report the DNA match, c) In families with several related victims, some laboratories firstly identified some victims and then unnecessarily used their genetic information to identify the remaining victims within the family, d) some laboratories did not correctly use "prior odds" values for the Bayesian treatment of the episode for both post-mortem/post-mortem re-associations as well as the ante-mortem/post-mortem comparisons to evaluate the probability of identity. For some of the above reasons, certain laboratories failed to identify some victims. This simulated "DNA-led" identification exercise may help forensic genetic laboratories to gain experience and expertize for DVI or MPI in using genetic data and comparing their own results with the ones in this collaborative exercise.

引用

页数：13

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