Determination of vitamin K1 in blood serum by high-performance liquid chromatography

被引：0

作者：

Sokolova, O. V. ^{[1
]}

Potapova, I. A. ^{[1
]}

机构：

[1] Nizhny Novgorod Res Inst Hyg & Occupat Pathol, Ul Semashko 20, Nizhnii Novgorod 603950, Russia

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2016年 / 71卷 / 07期

关键词：

vitamin K-1; HPLC; liquid extraction; blood serum;

D O I：

10.1134/S1061934816070157

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is proposed for the determination of vitamin K-1 concentration in blood serum using HPLC on a Gemini C18 (250 mm x 4.6 mm ID, 5 mu m) column and elution with a methanol-acetonitrile-dichloromethane (45 : 50 : 5, v/v) mobile phase at a flow rate of 1 mL/min and detection at 248 nm. The limit of detection for the vitamin is 0.5 ng/mL and relative error is 19%. The volume of sample in the column is 100 mu L. The method has been applied to the evaluation of vitamin K-1 deficit in a human body in medical practice.

引用

页码：737 / 740

页数：4

共 8 条

[1]

Bremener S. M., 1966, VITAMINY IKH KLIN PR

[2]

CHAM BE, 1989, CLIN CHEM, V35, P2285

[3] The chemical and biological activities of quinones: overview and implications in analytical detection [J].