NMR as a tool to investigate the structure, dynamics and function of membrane proteins

被引：77
|
作者：
Liang, Binyong [1 ,2 ]
Tamm, Lukas K. [1 ,2 ]
机构：
[1] Univ Virginia, Sch Med, Ctr Membrane & Cell Physiol, Charlottesville, VA 22908 USA
[2] Univ Virginia, Sch Med, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2016年 / 23卷 / 06期
关键词：
SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; HIGH-RESOLUTION STRUCTURE; SENSORY RHODOPSIN II; OUTER-MEMBRANE; BETA(2)-ADRENERGIC RECEPTOR; LIPID-BILAYERS; PSEUDOMONAS-AERUGINOSA; PHOSPHOLIPID-BILAYERS; CONFORMATIONAL-CHANGE;
D O I：
10.1038/nsmb.3226
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Membrane-protein NMR occupies a unique niche for determining structures, assessing dynamics, examining folding, and studying the binding of lipids, ligands and drugs to membrane proteins. However, NMR analyses of membrane proteins also face special challenges that are not encountered with soluble proteins, including sample preparation, size limitation, spectral crowding and sparse data accumulation. This Perspective provides a snapshot of current achievements, future opportunities and possible limitations in this rapidly developing field.
引用
收藏
页码：468 / 474
页数：7
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