NMR as a tool to investigate the structure, dynamics and function of membrane proteins

被引：77
|
作者：
Liang, Binyong [1 ,2 ]
Tamm, Lukas K. [1 ,2 ]
机构：
[1] Univ Virginia, Sch Med, Ctr Membrane & Cell Physiol, Charlottesville, VA 22908 USA
[2] Univ Virginia, Sch Med, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2016年 / 23卷 / 06期
关键词：
SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; HIGH-RESOLUTION STRUCTURE; SENSORY RHODOPSIN II; OUTER-MEMBRANE; BETA(2)-ADRENERGIC RECEPTOR; LIPID-BILAYERS; PSEUDOMONAS-AERUGINOSA; PHOSPHOLIPID-BILAYERS; CONFORMATIONAL-CHANGE;
D O I：
10.1038/nsmb.3226
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Membrane-protein NMR occupies a unique niche for determining structures, assessing dynamics, examining folding, and studying the binding of lipids, ligands and drugs to membrane proteins. However, NMR analyses of membrane proteins also face special challenges that are not encountered with soluble proteins, including sample preparation, size limitation, spectral crowding and sparse data accumulation. This Perspective provides a snapshot of current achievements, future opportunities and possible limitations in this rapidly developing field.
引用
收藏
页码：468 / 474
页数：7
相关论文
共 50 条
  • [1] NMR as a tool to investigate the structure, dynamics and function of membrane proteins
    Binyong Liang
    Lukas K Tamm
    Nature Structural & Molecular Biology, 2016, 23 : 468 - 474
  • [2] Conditional Membrane Proteins: Solution NMR Studies of Structure, Dynamics, and Function
    Yang, Yuan
    Morales, Krystal A.
    Stewart, Mikaela D.
    Igumenova, Tatyana I.
    EMAGRES, 2015, 4 (04): : 767 - 777
  • [3] NMR structure and function of membrane proteins in membranes
    Marassi, Francesca
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 254
  • [4] Membrane Proteins: Function, Structure, and Dynamics
    Senju, Yosuke
    Suetsugu, Shiro
    MEMBRANES, 2023, 13 (12)
  • [5] Diffusion NMR: A Tool to Investigate the Dynamics of Organic Systems
    Stait-Gardner, Timothy
    Tones, Allan
    Zubkov, Mikhail
    Willis, Scott A.
    Zheng, Gang
    Price, William S.
    CURRENT ORGANIC CHEMISTRY, 2018, 22 (08) : 758 - 768
  • [6] Structure and Dynamics of Membrane Proteins from Solid-State NMR
    Mandala, Venkata S.
    Williams, Jonathan K.
    Hong, Mei
    ANNUAL REVIEW OF BIOPHYSICS, VOL 47, 2018, 47 : 201 - 222
  • [7] NMR methods for structure-function investigation of membrane proteins and their ligands
    Patching, Simon G.
    Middleton, David A.
    Kalverda, Arnout
    Gowdy, James
    Baldwin, Stephen A.
    Phillips-Jones, Mary K.
    Levitt, Malcolm H.
    Homans, Steve W.
    Henderson, Peter J. F.
    JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, 2012, 55 (03): : 132 - 133
  • [8] Lipid Regulation of Structure, Dynamics, Energetics, and Function of Membrane Proteins
    Tajkhorshid, Emad
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2019, 48 : S63 - S63
  • [9] Solid-state NMR approaches for probing the structure and dynamics of membrane proteins
    Smith, SO
    BIOPHYSICAL JOURNAL, 2005, 88 (01) : 542A - 542A
  • [10] Towards a native environment: structure and function of membrane proteins in lipid bilayers by NMR
    Xue, Kai
    Movellan, Kumar Tekwani
    Zhang, Xizhou Cecily
    Najbauer, Eszter E.
    Forster, Marcel C.
    Becker, Stefan
    Andreas, Loren B.
    CHEMICAL SCIENCE, 2021, 12 (43) : 14332 - 14342
12345