Molecular typing of Treponema pallidum strains from patients with neurosyphilis in Pretoria, South Africa

被引：41

作者：

Molepo, J. ^{[1
]}

Pillay, A. ^{[1
]}

Weber, B. ^{[1
]}

Morse, S. A. ^{[1
]}

Hoosen, A. A. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div STD Prevent, Lab Reference, Res Branch, Atlanta, GA 30333 USA

来源：

SEXUALLY TRANSMITTED INFECTIONS | 2007年 / 83卷 / 03期

关键词：

D O I：

10.1136/sti.2006.023895

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objective: To evaluate the molecular typing system for Treponema pallidum using cerebrospinal fluid ( CSF) specimens obtained from patients with neurosyphilis in Pretoria, South Africa. Methods: CSF specimens were collected from 32 men and 18 women with suspected late neurosyphilis. Typing of T pallidum involved PCR amplification and restriction analysis of the tpr E, G and J genes and determination of the number of 60 base pair tandem repeats within the arp gene by PCR amplification. Results: Of 13 typeable specimens, 4 strain types were identified: 2i, 3e, 14a and 17e. Subtype 14a was identified in 7 specimens ( 53.8%), subtype 3e in 4 specimens ( 30.7%) and subtypes 17e and 2i in 1 specimen ( 7.6%) each. Conclusions: This study shows that the typing system can be applied to specimens which may contain low numbers of spirochaetes such as CSF.

引用

页码：189 / 192

页数：4

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