Tea Tree Oil Nanoemulsion Potentiates Antibiotics against Multidrug-Resistant Escherichia coli

被引：16
作者：
Wei, Simin [1 ,2 ,3 ]
Tian, Qiming [1 ,2 ,3 ]
Zhao, Xin [1 ,2 ,3 ]
Liu, Xiaopan [1 ,2 ,3 ]
Husien, Hosameldeen Mohamed [1 ,2 ,3 ,4 ]
Liu, Mingjiang [1 ,2 ,3 ]
Bo, Ruonan [1 ,2 ,3 ]
Li, Jingui [1 ,2 ,3 ]
机构：
[1] Yangzhou Univ, Sch Vet Med, Yangzhou 225009, Peoples R China
[2] Jiangsu Co Innovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China
[3] Yangzhou Univ, Joint Int Res Lab Agr & Agri Prod Safety, Minist Educ China, Yangzhou 225009, Jiangsu, Peoples R China
[4] Albutana Univ, Coll Vet Med, Rufaa 22217, Al Jazirah, Sudan
来源：
ACS INFECTIOUS DISEASES | 2022年 / 8卷 / 08期
基金：
中国国家自然科学基金;
关键词：
multidrug-resistant; tea tree oil; nanoemulsion; antibiotics; combination; EFFLUX PUMP; IN-VIVO; ACCUMULATION; TETRACYCLINES; COMBINATIONS; INHIBITION; BROAD;
D O I：
10.1021/acsinfecdis.2c00223
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Extensive efforts are underway to overcome the rising prevalence of antibiotic resistance. Combination therapy may be a potential method to treat multidrug-resistant (MDR) bacterial infections. In this study, tea tree essential oil (TTO) nanoemulsion (nanoTTO) was used in combination with antibiotics to kill microbes. Results showed that nanoTTO enhanced the activities of multiple antibiotics against MDR Escherichia coli (E. coli), and its antimicrobial activity was not changed against bacteria that were cultured in the presence of nanoTTO for 30 passages. Further studies to visualize and quantify intracellular antibiotics concentrations identified that nanoTTO increased the drug accumulation in MDR E. coli by disrupting outer and inner membranes and inhibiting the AcrAB-TolC efflux pump involved in membrane permeability. In addition, nanoTTO was effective in enhancing antibiotic efficacy in the Galleria mellonella infection model and mouse peritonitis model, suggesting a potential strategy against MDR bacterial infections.
引用
收藏
页码：1618 / 1626
页数：9
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