Nanoliposomes carrying HER2/neu-derived peptide AE36 with CpG-ODN exhibit therapeutic and prophylactic activities in a mice TUBO model of breast cancer

被引：20

作者：

Barati, Nastaran ^{[1
,2
]}

Nikpoor, Amin Reza ^{[3
]}

Razazan, Atefeh ^{[4
]}

Mosaffa, Fatemeh ^{[2
,5
]}

Badiee, Ali ^{[1
,5
]}

Arab, Atefeh ^{[1
,2
]}

Gholizadeh, Zahra ^{[3
]}

Behravan, Javad ^{[2
,5
]}

Jaafari, Mahmoud Reza ^{[1
,2
,5
]}

机构：

[1] Mashhad Univ Med Sci, Nanotechnol Res Ctr, Mashhad, Iran

[2] Mashhad Univ Med Sci, Biotechnol Res Ctr, Mashhad, Iran

[3] Mashhad Univ Med Sci, Sch Med, Dept Immunol, Mashhad, Iran

[4] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Mol Med, Tehran, Iran

[5] Mashhad Univ Med Sci, Sch Pharm, Mashhad, Iran

来源：

IMMUNOLOGY LETTERS | 2017年 / 190卷

关键词：

HER2/neu-derived peptide; Nanoliposomes; CpG; Vaccine; Cancer; PH-SENSITIVE LIPOSOMES; CD8(+) T-CELL; EFFECTIVE INDUCTION; ESTABLISHED TUMORS; SOLUBLE-PROTEINS; VACCINE DELIVERY; IMMUNE-RESPONSE; PROSTATE-CANCER; BALB/C MICE; PHASE;

D O I：

10.1016/j.imlet.2017.07.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study was designed to prepare and characterize nanoliposomal vaccine formulation encapsulating AE36 HER2/neu-derived peptide with or without CpG and evaluate the immunologic and therapeutic responses of that in BALB/c mice model of Her2 overexpressing breast cancer. AE36 was encapsulated in liposomes composed of DOTAP, DOPE and Cholesterol (DDC) or DD with. The formulations could induce both CD8+ and CD4+ responses and stimulate production of cytokines which was detected by Enzyme-linked immunospot assay (ELISpot) kits, cytotoxicity test and intracellular cytokine assay by flow cytometry. The formulation showed both therapeutic and prophylactic effects in BALB/c mice bearing Her2(+) breast cancer. DDC + CpG showed the best effect in prophylactic study and DD + pG showed the best effect in therapeutic study, which both of them decreased the size of tumors significantly. The engineered nanoliposomes containing AE36 could be a candidate vaccine for the treatment or prophylaxis of HER2(+) breast cancer and merits further investigation.

引用

页码：108 / 117

页数：10

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