First molecular evidence of Babesia caballi and Theileria equi infections in horses in Cuba

被引：29

作者：

Alberto Diaz-Sanchez, Adrian ^{[1
]}

Pires, Marcus Sandes ^{[2
]}

Yrurzun Estrada, Carlos ^{[3
]}

Vega Canizares, Ernesto ^{[1
]}

del Castillo Dominguez, Sergio Luis ^{[1
]}

Cabezas-Cruz, Alejandro ^{[4
]}

Lobo Rivero, Evelyn ^{[1
]}

Henrique da Fonseca, Adivaldo ^{[2
]}

Luiz Massard, Carlos ^{[2
]}

Corona-Gonzalez, Belkis ^{[1
,5
]}

机构：

[1] Natl Ctr Anim & Plant Hlth CENSA, Carretera Tapaste & Autopista Nacl Km 22 1-2, San Jose De Las Lajas 32700, Mayabeque, Cuba

[2] Fed Rural Univ Rio De Janeiro UFRRJ, Dept Epidemiol & Publ Hlth, BR 465,Km 7, BR-23890000 Seropedica, RJ, Brazil

[3] Agr Univ Habana UNAH, Vet Med Coll, Dept Anim Prevent, Carretera Tapaste & Autopista Nacl Km 22 1-2, San Jose De Las Lajas, Mayabeque, Cuba

[4] Univ Paris Est, Ecole Natl Vet Alfort, INRA, UMR,BIPAR,ANSES, F-94700 Maisons Alfort, France

[5] Natl Ctr Anim & Plant Hlth CENSA, Anim Hlth Div, Carretera Tapaste & Autopista Nacl Km 22 1-2, San Jose De Las Lajas 32700, Mayabeque, Cuba

来源：

PARASITOLOGY RESEARCH | 2018年 / 117卷 / 10期

关键词：

Equine piroplasmosis; Babesia caballi; Theileria equi; nPCR; 18S rRNA; PIROPLASMOSIS; TRANSMISSION; PREVALENCE; ALIGNMENT;

D O I：

10.1007/s00436-018-6005-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Equine piroplasmosis is a disease of Equidae, including horses, donkeys, mules, and zebras, caused by either Theileria equi or Babesia caballi. This disease represents a serious problem for the horse industry and its control is critical for the international trade of horses. The objective of the present study was to detect B. caballi and T. equi infections in horses reared in western Cuba. Blood samples from 100 horses were tested for the presence of piroplasms by using Giemsa-stained blood smears and nested PCR (nPCR) assays targeting merozoite antigen genes of B. caballi (bc48) and T. equi (ema-1). All animals were inspected for the detection of tick infestation and tick specimens were collected for species identification. Erythrocyte inclusions were observed in 13 (13%) of the analyzed samples. nPCR analysis showed that 25 (25%) samples were positive for B. caballi, 73 (73%) for T. equi, and 20 (20%) showed dual infections. Only one tick species was found infesting horses, Dermacentor nitens. In addition, three nearly full-length sequences of T. equi 18S rRNA gene were obtained and subjected to phylogenetic analyses. This study reports a high prevalence of T. equi and B. caballi single and coinfections in horses in western Cuba. Molecular analysis of the 18S rRNA gene of T. equi suggested that different genotypes of this hemoparasite circulate in Cuba. To the best of our knowledge, this is the first report describing the molecular detection of B. caballi and T. equi in horses in Cuba.

引用

页码：3109 / 3118

页数：10

共 45 条

[1] Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood [J].