Quantum dot-based western blot for sensitive detection of pig serum antibody to actinobacillus pleuropneumoniae

被引：0

作者：

Cismileanu, Ana ^{[1
]}

Sima, Cornelia ^{[2
]}

Grigoriti, Constantin ^{[2
]}

机构：

[1] Inst Pasteur, Bucharest, Romania

[2] Natl Inst Laser, Plasma & Radiat Phys, Bucharest, Romania

来源：

ROMOPTO 2006: EIGHTH CONFERENCE ON OPTICS | 2007年 / 6785卷

关键词：

quantum dots; bioconjugation; Actinobacillus pleuropneumoniae; nanoparticles; CELLS;

D O I：

10.1117/12.756829

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A quantum dot - immunoglobulin conjugate specific for pig IgG, was obtained by carbodiimide chemistry. We used a Western blot technique for detecting specific antibodies against Actinobacillus pleuropneumoniae (A. pp), which cause porcine pleuropneumonia. The antigen used in this technique was Apx haemolysin which is an important virulence factor of A. pp and it induces protective immunity in vaccined pigs. The detection on Western blot membrane was possible at 1150 dilution of quantum dot conjugate at a dilution of pig serum till 1/6400. The results for pig serum demonstrated a higher sensitivity of QD-based Western blot technique for the presence of antibodies specific for Apx haemolysin in comparison with similar classical techniques (with coloured substrate for enzyme present in secondary antibody conjugate).

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