Patched 1 conditional null allele in mice

被引：87

作者：

Ellis, T

Smyth, I

Riley, E

Graham, S

Elliot, K

Narang, M

Kay, GF

Wicking, C

Wainwright, B

机构：

[1] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

[2] Cooperat Res Ctr Discovery Genes Common Human Dis, Richmond, Vic, Australia

[3] Murdoch Childrens Res Inst, Melbourne, Vic, Australia

[4] Queensland Inst Med Res, Brisbane, Qld 4006, Australia

来源：

GENESIS | 2003年 / 36卷 / 03期

关键词：

patched; Ptc; conditional knockout; developmental anomalies; cancer; Cre : lox;

D O I：

10.1002/gene.10208

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The patched gene (Ptc) is a member of the hedgehog signaling pathway which plays a central role in the development of many invertebrate and vertebrate tissues. In addition, Ptc and a number of other pathway members are mutated in some common human cancers. Patched is the receptor for the hedgehog ligand and in the mouse ablation of the Ptc gene leads to developmental defects and an embryonic lethal phenotype. Here we describe a conditional Ptc allele in mice which will have utility for the temporospatial ablation of Ptc function. genesis 36:158-161, 2003. (C) 2003 Wiley-Liss, Inc.

引用

收藏

页码：158 / 161

页数：4

相关论文

共 23 条

[1] Mice lacking the vascular endothelial growth factor-B gene (Vegfb) have smaller hearts, dysfunctional coronary vasculature, and impaired recovery from cardiac ischemia [J].

Bellomo, D ;

Headrick, JP ;

Silins, GU ;

Paterson, CA ;

Thomas, PS ;

Gartside, M ;

Mould, A ;

Cahill, MM ;

Tonks, ID ;

Grimmond, SM ;

Townson, S ;

Wells, C ;

Little, M ;

Cummings, MC ;

Hayward, NK ;

Kay, GF .

CIRCULATION RESEARCH, 2000, 86 (02) :E29-E35

[2] Small molecule modulation of Smoothened activity [J].

Chen, JK ;

Taipale, J ;

Young, KE ;

Maiti, T ;

Beachy, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (22) :14071-14076

[3] Hedgehog induces opposite changes in turnover and subcellular localization of patched and smoothened [J].

Denef, N ;

Neubüser, D ;

Perez, L ;

Cohen, SM .

CELL, 2000, 102 (04) :521-531

[4] Sonic hedgehog protein signals not as a hydrolytic enzyme but as an apparent ligand for Patched [J].

Fuse, N ;

Maiti, T ;

Wang, BL ;

Porter, JA ;

Hall, TMT ;

Leahy, DJ ;

Beachy, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :10992-10999

[5] Conservation of the hedgehog/patched signaling pathway from flies to mice: Induction of a mouse patched gene by Hedgehog [J].

Goodrich, LV ;

Johnson, RL ;

Milenkovic, L ;

McMahon, JA ;

Scott, MP .

GENES & DEVELOPMENT, 1996, 10 (03) :301-312

[6] Altered neural cell fates and medulloblastoma in mouse patched mutants [J].

Goodrich, LV ;

Milenkovic, L ;

Higgins, KM ;

Scott, MP .

SCIENCE, 1997, 277 (5329) :1109-1113

[7] NEVOID BASAL-CELL CARCINOMA SYNDROME [J].

GORLIN, RJ .

DERMATOLOGIC CLINICS, 1995, 13 (01) :113-125

[8] Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome [J].

Hahn, H ;

Wicking, C ;

Zaphiropoulos, PG ;

Gailani, MR ;

Shanley, S ;

Chidambaram, A ;

Vorechovsky, I ;

Holmberg, E ;

Unden, AB ;

Gillies, S ;

Negus, K ;

Smyth, I ;

Pressman, C ;

Leffell, DJ ;

Gerrard, B ;

Goldstein, AM ;

Dean, M ;

Toftgard, R ;

ChenevixTrench, G ;

Wainwright, B ;

Bale, AE .

CELL, 1996, 85 (06) :841-851

[9] Patched represses the Hedgehog signalling pathway by promoting modification of the Smoothened protein [J].

Ingham, PW ;

Nystedt, S ;

Nakano, Y ;

Brown, W ;

Stark, D ;

van den Heuvel, M ;

Taylor, AM .

CURRENT BIOLOGY, 2000, 10 (20) :1315-1318

[10] Human homolog of patched, a candidate gene for the basal cell nevus syndrome [J].

Johnson, RL ;

Rothman, AL ;

Xie, JW ;

Goodrich, LV ;

Bare, JW ;

Bonifas, JM ;

Quinn, AG ;

Myers, RM ;

Cox, DR ;

Epstein, EH ;

Scott, MP .

SCIENCE, 1996, 272 (5268) :1668-1671