Expression and secretion of Bluetongue virus serotype 8 (BTV-8)VP2 outer capsid protein by mammalian cells

被引：7

作者：

Capocefalo, A. ^{[1
]}

Franceschi, V. ^{[1
]}

Mertens, P. P. ^{[2
]}

Castillo-Olivares, J. ^{[2
]}

Cavirani, S. ^{[1
]}

Di Lonardo, E. ^{[1
]}

Leni, Z. ^{[1
]}

Donofrio, G. ^{[1
]}

机构：

[1] Univ Parma, Dipartimento Salute Anim, Fac Med Vet, I-43100 Parma, Italy

[2] Pirbright Lab, Inst Anim Hlth, Surrey, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2010年 / 169卷 / 02期

关键词：

Bluetongue; Viral protein 2; Expression; Secretion; ANTIBODIES; VACCINES;

D O I：

10.1016/j.jviromet.2010.08.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

VP2 is the outermost Bluetongue virus (BTV) antigenic protein, forming triskelion motifs on the virion surface. Although VP2 has been expressed successfully through many systems, its paracrine expression as a soluble form by mammalian cells represents a difficult task In the present paper two fragments of VP2 have been expressed successfully into the medium of transiently transfected mammalian cells through a fusion peptides strategy. The crude conditioned medium containing the secreted peptide could be employed for immunodiagnostic assay development or vaccine purposes. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：420 / 424

页数：5

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