Changes in the composition of anionic membrane phospholipids influence protein secretion and cell envelope biogenesis in Escherichia coli

被引：3
|
作者：
Anisimova, EV [1 ]
Badyakina, AO [1 ]
Vasil'eva, NV [1 ]
Nesmeyanova, MA [1 ]
机构：
[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Oblast, Russia
来源：
MICROBIOLOGY | 2005年 / 74卷 / 02期
基金：
俄罗斯基础研究基金会;
关键词：
Escherichia coli; alkaline phosphatase; secretion; phospholipids; outer membrane;
D O I：
10.1007/s11021-005-0044-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The secretion of alkaline phosphatase (PhoA) and peculiarities of biogenesis of the cell envelope were studied in Escherichia coli strains HD30/pHD102 and HDL11 with controlled synthesis of the anionic phospholipids, phosphatidylglycerol and cardiolipin. Inactivation of the pgsA gene responsible for the synthesis of anionic phospholipids or changes in the regulation of its expression by an environmental factor caused changes in the metabolism and composition of membrane phospholipids, which resulted in a decrease in the secretion of alkaline phosphatase through the cytoplasmic membrane and an increase in PhoA secretion from the periplasm into the culture medium. An increase was observed in exopolysaccharide secretion, as well as a decrease in the contents of the outer membrane lipopolysaccharides and lipopolyproteins, which determine its barrier properties. The results obtained show that anionic phospholipids play a significant role in protein secretion and are probably involved in the interrelation between protein secretion and biogenesis of cell envelope components.
引用
收藏
页码：147 / 152
页数：6
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