Introduction: Lumbar spondylolysis is a common injury in badminton players' career. The occurrence of lumbar spondylolysis will affect athletes' competitive level, physical and mental health, which requires attention in daily care and treatment. Methods: In this paper, badminton players' lumbar spondylolysis was analyzed based on biomechanical research method. Besides, three-dimensional activity range of upper spine in biological model and lumbar spondylolysis upper spinal stability was studied and discussed, with the aim to provide a practical reference for trauma therapy of badminton players' lumbar spondylolysis. In this study, 3000 cases of adults' fresh lumbar specimens were selected and the cut off L5 bilateral spondylolysis formed as the test model. The specimen was applied with flexion, extension, lateral bending and rotation of 10 Nm torque before (normal) and after modeling (lumbar spondylolysis group) via three-dimensional movement experimental mechanism of the spine. Results: From the results obtained, the flexion, extension and rotation for upper spine (L4) spondylolysis group specimens was 12.70 degrees, 4.80 degrees and 3.71 degrees respectively while the average values for normal group model were 10.40 degrees, 3.70 degrees and 2.78 degrees respectively. Comparison of both the test data demonstrated significant statistical difference. The results were higher in lumbar spondylolysis group with the value of 22.1%, 29.4% and 33.8% respectively. However there is no significant difference between the average value of flexion activities for spondylolysis group (7.74 degrees) and normal group (7.67 degrees) Spondylolysis spine (1.5) motion range of flexion, extension and rotation gave values of 12.69 degrees, 6.29 degrees, 4.10 degrees, while average value of normal group was 10.12 degrees, 5.19 degrees, 3.01 degrees respectively, with significant differences between the groups, reflecting P value less than 0.01. Spondylolysis group values increased by 25.4%, 44.1% and 36.4% while range of flexion activities for spondylolysis group was 6.12 degrees and 6.06 degrees for normal group. The difference between the groups was not obvious therefore it's not statistically significant. Conclusions: From the above studies, specific conclusion is that lumbar spondylolysis will adversely affect the badminton players' upper spine stability. It should also take be noted that, some of the badminton players might suffer from instability of upper spine in flexion, extension and rotation, which has a negative impact on athletes' competitive level and career.