Application of Spiroplasma melliferum Proteogenomic Profiling for the Discovery of Virulence Factors and Pathogenicity Mechanisms in Host-associated Spiroplasmas

被引：36

作者：

Alexeev, Dmitry ^{[1
,2
]}

Kostrjukova, Elena ^{[1
]}

Aliper, Alexander ^{[1
]}

Popenko, Anna ^{[1
]}

Bazaleev, Nikolay ^{[1
]}

Tyakht, Alexander ^{[1
]}

Selezneva, Oksana ^{[1
,3
]}

Akopian, Tatyana ^{[1
]}

Prichodko, Elena ^{[1
]}

Kondratov, Ilya ^{[1
]}

Chukin, Mikhail ^{[1
]}

Demina, Irina ^{[1
]}

Galyamina, Maria ^{[1
]}

Kamashev, Dmitri ^{[1
,3
]}

Vanyushkina, Anna ^{[1
,3
]}

Ladygina, Valentina ^{[1
]}

Levitskii, Sergei ^{[1
,3
]}

Lazarev, Vasily ^{[1
,3
]}

Govorun, Vadim ^{[1
,3
,4
]}

机构：

[1] Russian Inst Physicochem Med, Moscow, Russia

[2] Moscow Inst Phys & Technol Bioinformat Dolgoprudn, Moscow 117303, Russia

[3] Russian Res Ctr, Kurchatov Inst, Moscow 123182, Russia

[4] MM Shemyakin Yu A Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

JOURNAL OF PROTEOME RESEARCH | 2012年 / 11卷 / 01期

关键词：

Spiroplasma; proteogenomics; metabolomics; virulence factors; systems approach; VECTOR CIRCULIFER-HAEMATOCEPS; GENOME-REDUCED BACTERIUM; CORN STUNT SPIROPLASMA; FLAVESCENCE DOREE MLO; PERITROPHIC MATRIX; CLOSTRIDIUM-PERFRINGENS; EUSCELIDIUS-VARIEGATUS; PHYLOGENETIC ANALYSIS; CHITIN DEACETYLASE; BINDING-PROTEIN;

D O I：

10.1021/pr2008626

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.

引用

页码：224 / 236

页数：13

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