A high-performance liquid chromatography method to measure tocopherols in assai pulp (Euterpe oleracea)

被引：22
作者：
Darnet, Sylvain [1 ]
Serra, Josilene L. [2 ]
da Cruz Rodrigues, Antonio Manoel [2 ]
Meller da Silva, Luiza H. [2 ]
机构：
[1] Fed Univ Para, ICB, BR-66075900 Belem, Para, Brazil
[2] Fed Univ Para, ITEC, Programa Posgrad Ciencias & Tecnol Alimentos, Inst Tecnol, BR-66075900 Belem, Para, Brazil
来源：
FOOD RESEARCH INTERNATIONAL | 2011年 / 44卷 / 07期
关键词：
Food analysis; HPLC; alpha-tocopherol; Vitamin E; Assai; Euterpe oleracea; VITAMIN-E; ALPHA-TOCOPHEROL; FATTY-ACID; MART; ACAI; FRUITS; ANTIOXIDANT; CAROTENOIDS; OIL; TOCOTRIENOL; CAPACITIES;
D O I：
10.1016/j.foodres.2010.12.039
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A high-performance liquid chromatography (HPLC) method for the laboratory scale determination of the tocopherol content of assai pulp is reported. This method includes the description of microscale saponification and extraction with diethyl ether. The optimized conditions for reversed-phase HPLC with UV and fluorescence detection were as follows: 250 mm x 4.60 mm Gemini C18 column with 5 mu m particles, 28 degrees C column temperature, 95:5 (v v I) methanol:water mobile phase, 1.0 mL min(-1) flow rate and 2.6% method precision for a-tocopherol. To determine the alpha-, beta-, gamma- and delta-tocopherol content of fresh pulp, the analyzed assai pulp was collected from three different regions of production in the Amazonian estuary. This study revealed that assai pulp is rich in vitamin E (394.31 mu g g(-1) dry matter of alpha-tocopherol), confirming the nutritional properties of assai. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2107 / 2111
页数：5
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