Clinical impact of LncRNA XIST and LncRNA NEAT1 for diagnosis of high-risk group breast cancer patients

被引:22
作者
Swellam, Menha [1 ]
El Magdoub, Hekmat M. [2 ]
Shawki, May A. [3 ]
Adel, Marwa [3 ]
Hefny, Mona M. [4 ]
El-Shazly, Samar Sami [5 ]
机构
[1] Natl Res Ctr, Ctr Excellence Adv Sci, Biochem Dept, High Throughput Mol & Genet Lab,Genet Engn & Biot, Giza, Egypt
[2] Misr Int Univ, Fac Pharm, Biochem Dept, Cairo, Egypt
[3] Ain Shams Univ, Fac Pharm, Clin Pharm Dept, Cairo, Egypt
[4] Ain Shams Univ, Fac Med, Med Biochem Dept, Cairo, Egypt
[5] Natl Canc Inst, Clin Pathol Dept, Cairo, Egypt
关键词
lncRNA; NEAT1; XIST; Noninvasive; Breast cancer diagnosis; Clinicopathological factors; LONG NONCODING RNAS; EXPRESSION; BIOMARKERS; REGULATOR; ROLES; WOMEN; SERUM;
D O I
10.1016/j.currproblcancer.2021.100709
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Long noncoding RNAs (lncRNAs) are evolving as contributing biomarkers for many diseases. Among these lncRNAs, X inactive-specific transcript (XIST), and nuclear paraspeckle assembly transcript 1 (NEAT1) were studied as undesirable upregulated nucleic acid markers for unfavorable prognosis of cancer. The authors aimed to investigate their role as diagnostic markers for breast cancer (BC) patients with high-risk factors. Serum samples were obtained from BC patients (n = 121), patients with benign breast lesions (n = 35), and healthy volunteers (n = 22). Assessment of lncRNA XIST, and lncRNA NEAT1 expression was performed using real time PCR. Expression levels of the investigated lncRNAs were significantly higher in BC patients as compared to the other groups. Both lncRNAs were significantly correlated with BC laterality, lymph node involvement, and clinical stages. LncRNA NEAT1 reported a significant aberrant expression with pathological types, histological grading and, hormonal status. The sensitivity of lncRNA NEAT1 was superior for detection of BC with high risk-factors as compared to lncRNA XIST. In conclusion, the detection of lncRNAs in body fluids has demonstrated a significant importance for detecting BC patients with high-risk factors, and was related to hormonal receptors, thus may be used for determining the direction of treatment strategy. (c) 2021 Elsevier Inc. All rights reserved.
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页数:10
相关论文
共 41 条
  • [1] Arkoob K., 2010, Eastern Mediterranean Health Journal, V16, P1032
  • [2] Expression Analysis of MALAT1, GAS5, SRA, and NEAT1 lncRNAs in Breast Cancer Tissues from Young Women and Women over 45 Years of Age
    Arshi, Asghar
    Sharifi, Fatemeh Sadat
    Ghahfarokhi, Milad Khorramian
    Faghih, Zahra
    Doosti, Abbas
    Ostovari, Sara
    Maymand, Elham Mahmoudi
    Seno, Mohammad Mahdi Ghahramani
    [J]. MOLECULAR THERAPY-NUCLEIC ACIDS, 2018, 12 : 751 - 757
  • [3] Long non-coding RNAs and their implications in cancer epigenetics
    Beckedorff, Felipe C.
    Amaral, Murilo Sena
    Deocesano-Pereira, Carlos
    Verjovski-Almeida, Sergio
    [J]. BIOSCIENCE REPORTS, 2013, 33 : 667 - 675
  • [4] Mammographic density and the risk and detection of breast cancer
    Boyd, Norman F.
    Guo, Helen
    Martin, Lisa J.
    Sun, Limei
    Stone, Jennifer
    Fishell, Eve
    Jong, Roberta A.
    Hislop, Greg
    Chiarelli, Anna
    Minkin, Salomon
    Yaffe, Martin J.
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2007, 356 (03) : 227 - 236
  • [5] Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1 leading to cancer cell survival
    Choudhry, H.
    Albukhari, A.
    Morotti, M.
    Haider, S.
    Moralli, D.
    Smythies, J.
    Schoedel, J.
    Green, C. M.
    Camps, C.
    Buffa, F.
    Ratcliffe, P.
    Ragoussis, J.
    Harris, A. L.
    Mole, D. R.
    [J]. ONCOGENE, 2015, 34 (34) : 4482 - 4490
  • [6] Urban-rural differences in breast cancer incidence in Egypt (1999-2006)
    Dey, Subhojit
    Soliman, Amr S.
    Hablas, Ahmad
    Seifeldein, Ibrahim A.
    Ismail, Kadry
    Ramadan, Mohamed
    El-Hamzawy, Hesham
    Wilson, Mark L.
    Banerjee, Mousumi
    Boffetta, Paolo
    Harford, Joe
    Merajver, Sofia D.
    [J]. BREAST, 2010, 19 (05) : 417 - 423
  • [7] Receptor-Defined Subtypes of Breast Cancer in Indigenous Populations in Africa: A Systematic Review and Meta-Analysis
    Eng, Amanda
    McCormack, Valerie
    dos-Santos-Silva, Isabel
    [J]. PLOS MEDICINE, 2014, 11 (09)
  • [8] Review: Regulation of the cancer epigenome by long non-coding RNAs
    Forrest, Megan E.
    Khalil, Ahmad M.
    [J]. CANCER LETTERS, 2017, 407 : 106 - 112
  • [9] The staging of cancer: A retrospective and prospective appraisal
    Greene, Frederick L.
    Sobin, Leslie H.
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2008, 58 (03) : 180 - 190
  • [10] A COMPARATIVE-STUDY OF THE PEROXIDASE-ANTIPEROXIDASE METHOD AND AN AVIDIN-BIOTIN COMPLEX METHOD FOR STUDYING POLYPEPTIDE HORMONES WITH RADIOIMMUNOASSAY ANTIBODIES
    HSU, SM
    RAINE, L
    FANGER, H
    [J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1981, 75 (05) : 734 - 738