Isolation of a galactose-specific lectin from Trichosanthes tricuspidatus root tuber

被引：0
|
作者：
Seshagirirao, K [1 ]
机构：
[1] Univ Hyderabad, Sch Life Sci, Dept Plant Sci, Appl Bot Lab, Hyderabad 500046, Andhra Pradesh, India
来源：
BIOCHEMICAL ARCHIVES | 1999年 / 15卷 / 01期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A lectin from the root tuber of Trichosanthes tricuspidatus has been isolated by affinity chromatography on cross-linked guar gum column. In SDS-PAGE the purified lectin (Trichosanthes tricuspidatus tuber agglutinin: TTTA) resolved as a single band at M-r 67 and 28 kDa in the absence and presence of beta-mercaptoethanol, respectively. It is a glycoprotein with 2.7 % of carbohydrate content. It agglutinated rabbit and human ABO erythrocytes. The agglutination was inhibited galactose and galactose containing sugars with increased beta-anomeric specificity.
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页码：25 / 29
页数：5
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