Two-step enzymatic synthesis of UDP-N-acetylgalactosamine

被引：27

作者：

Bourgeaux, V

Piller, F

Piller, V

机构：

[1] Univ Orleans, Ctr Biophys Mol, CNRS, UPR4301, F-45071 Orleans, France

[2] INSERM, F-45071 Orleans, France

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2005年 / 15卷 / 24期

关键词：

UDP-GalNAc; enzymatic synthesis;

D O I：

10.1016/j.bmcl.2005.08.088

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

UDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. Both enzymes have been prepared in one step from I L cultures of transformed Escherichia coli and I he UDP-GalNAc produced has been purified by a simple procedure. The method described is a rapid ;and efficient means to produce UDP-GalNAc as well as analogues like UDP-N-azidoacetylgalactosamine (UDP-GalNAz). (c) 2005 Elsevier Ltd. All rights reserved.

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收藏

页码：5459 / 5462

页数：4

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